EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of human lympho blast plasma membrane 5' nucleotidase ec 3.1.3.5 by zinc


, : Regulation of human lympho blast plasma membrane 5' nucleotidase ec 3.1.3.5 by zinc. Journal of Biological Chemistry 257(22): 13544-13549

Human lymphoblasts lose > 90% of their plasma membrane 5'-nucleotidase activity within 24 h when grown in Zn-deficient (< 0.05 .mu.M Zn(II)) culture media prepared with either bovine serum albumin/fatty acids/transferrin or with extensively dialyzed fetal bovine serum as supplements. The loss of 5'-nucleotidase activity is accompanied by a decrease in substrate affinity. The inactivation of plasma membrane 5'-nucleotidase is temperature-dependent and requires the metabolically active cell. When isolated plasma membranes are incubated under the same conditions as the whole cells, they do not lose their 5'-nucleotidase activity. Supplementation of the Zn-deficient culture media with 10 .mu.M Zn prevents the loss of 5'-nucleotidase activity. Addition of 10 .mu.M Zn to Zn-deficient cells or plasma membranes prepared from cells with low 5'-nucleotidase activity due to Zn depletion leads to complete recovery of activity within 24 h. The recovery is not prevented by inhibition of protein synthesis. The reactivation of 5'-nucleotidase shows a dependence on temperature which is clearly distinct from the inactivation process. The addition of Co(II), Cu(II) or Cd(II) to Zn-deficient cultures leads to some activation of 5'-nucleotidase, but none of the divalent cations tested are nearly as effective as Zn. The observed changes in 5'-nucleotidase activity were not found in other divalent cation-requiring plasma membrane enzymes (ATPase, alkaline phosphatase). Apparently, plasma membrane 5'-nucleotidase can exist as an inactive apoenzyme and Zn plays a unique role in the expression of plasma membrane 5'-nucleotidase activity.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006288550

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Tathcioglu, T.; Gursel, G., 1996: Reliability of intrabreath method to measure transfer factor in patients with airflow limitation. European Respiratory Journal Supplement 9(23): 122S

Imseis H.; Mizell M.; Ranier J.; Hofmann L., 1987: Oncogene analysis of the lucke carcinoma and of early embryonic development in rana pipiens. American Zoologist 27(4): 144A

Turk, Zmago, 2001: Knochenbruchheilung und biochemische blutparameter bei kaninchen nach kunstlicher femurosteotomie im schwachen niederfrequenten magnetfeld. Aim of the study was to investigate the effect of magnetic fields on bone fracture healing and biochemical indices of bone turnover. Young male "White Newzealand Rabbits" were exposed after osteotomy of the femur of the hind-paw in narco...

Ling, I.T.; Wilson, R.J.M., 1988: Glucose-6-phosphate dehydrogenase activity of the malaria parasite Plasmodium falciparum. Schizonts of P. falciparum, grown either in normal or glucose-6-phosphate dehydrogenase (G6PDH) deficient human red cells, contained an electrophoretically slow-moving form of G6PDH. The slow mobility of the G6PDH in non-dissociating polyacrylamid...

Tan, L.T.; Zahra, M., 2008: Long-term survival and late toxicity after chemoradiotherapy for cervical cancer--the Addenbrooke's experience. To evaluate the long term cause-specific survival and late toxicity of chemoradiotherapy for carcinoma of the cervix treated outside research settings. Between May 1999 and April 2003, 74 patients with carcinoma of the cervix were treated with rad...

FUJIMOTO, T., 1963: On The Supravital Staining Of The Mitochondria Of Cultured Cells With Pinacyanole. Kaibogaku Zasshi. Journal of Anatomy 38: 185-187

Weiland, S.; Bertrand, D.; Leonard, S., 2000: Neuronal nicotinic acetylcholine receptors: From the gene to the disease. The neuronal nicotinic acetylcholine receptors are excitatory ligand-gated channels. Widely expressed throughout the peripheral and central nervous system, their properties depend upon their subunit composition. Furthermore, genetic studies have r...

Nesterenko, V.P.; Dubrovina, Z.V.; Belova, O.M., 1966: On the problem of the assimilability of strontium-90 from bread. Voprosy Pitaniia 25(5): 58-63

Geller, L.I.; Amatniak, A.G., 1989: A prognostic criterion for the course of chronic recurrent pancreatitis. Disorders in the blood kallikrein-kinin system presenting mainly with kallikrein elevation and accelerated rate of bradykinin release from kininogen, hyperhistaminemia and hyperserotoninemia may be considered natural for chronic recurrent pancreat...

Hildreth, D.I.; Crisp, D.J., 1976: A corrected formula for calculation of filtration rate of bivalve mollusks in an experimental flowing system. The filtration rate of Mytilus edulis was measured in a flowing system by estimating the particulate matter concentrations at the inflow (C1) and outflow (C2) of the experimental chamber and immediately surrounding the bivalve (C0). Filtration rat...