EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of human peripheral blood monocyte DR antigen expression in vitro by lymphokines and recombinant interferons


, : Regulation of human peripheral blood monocyte DR antigen expression in vitro by lymphokines and recombinant interferons. Journal of Clinical Investigation 73(2): 556-565

The in vitro regulation of adult human monocyte DR antigen expression was studied. Normally about 75% of freshly obtained human peripheral blood monocytes express DR antigens as determined by anti-DR and complement-mediated cytotoxicity assays. DR expression on monocytes in unfractionated peripheral blood mononuclear cell cultures persisted to variable degrees for up to 5 d of incubation. However, when the mononuclear cells were thoroughly depleted of nonadherent cells, cultured monocytes consistently exhibited progressively decreased DR expression over 2-5 d of incubation. Readdition of nonadherent cells to the adherent cell population prevented or delayed this decrease in monocyte DR antigen expression. Thus, monocyte DR expression diminished markedly during in vitro incubation; however, the presence of nonadherent cells somehow interfered with this process. In other experiments, peripheral adherent monocytes, which had been cultured for 2-3 d to reduce their DR expression, could be induced to reexpress DR antigens after 2 d of incubation with unpurified lymphokine-containing culture supernatants, recombinant human interferon-alpha, or recombinant human gamma interferon (IFN-gamma). The reinduction of DR expression on human monocytes by lymphokines was abrogated by an antiserum produced to the synthetic N-terminal amino acids of human IFN-gamma, indicating that IFN-gamma is the active mediator in the lymphokine-containing preparations. Monocytes cultured with lymphokines or recombinant interferons also could initiate a significantly greater mixed lymphocyte response than control monocytes. Thus, IFN-gamma-containing lymphokines and recombinant interferons are required to induce human monocyte DR expression and accessory cell capacity in vitro, since in their absence monocytes become DR antigen-deficient. Finally, incubation of unfractionated human mononuclear cells with anti-human IFN-gamma also promoted the loss of monocyte DR expression. These findings suggest that resting lymphocytes are probably capable of producing sufficient IFN-gamma in vitro to result in the maintenance of the monocyte DR phenotype.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006288575

PMID: 6230374

DOI: 10.1172/JCI111243

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Thomson, G. M., 1880: New Species of Crustacea from New Zealand. Annals of Natural History, 5(vi): 1-6

Robertson R.D.; Sarti D.; Crandall B.F., 1980: Pre natal diagnosis of hydrocephalus by ultrasound in a high risk patient. Clinical Research 28(1): 100A

Reed, Steven G.; Skeiky, Yasir A. W.; Dillon, Davin C.; Campos Neto, Antonio; Houghton, Raymond; Vedvick, Thomas S.; Twardzik, Daniel R.; Lodes, Michael J.; Hendrickson, Ronald C., 2003: Compounds and methods for immunotherapy and diagnosis of tuberculosis. Compounds and methods for inducing protective immunity against tuberculosis are disclosed. The compounds provided include polypeptides that contain at least one immunogenic portion of one or more M. tuberculosis proteins and DNA molecules encoding...

Scott, T. W.; White, I. G.; Annison, E. F., 1962: II. Glucose and acetate metabolism by ram, bull, dog and fowl spermatozoa. II. Ram, bull, dog and fowl spermatozoa metabolize acetate and glucose, but there are species differences. Ram and bull spermatozoa oxidize acetate in preference to glucose, but with dog spermatozoa the reverse is true. The preferential oxidation...

Elrod K.; Jackson W.J.; Buccafusco J.J., 1987: Nicotine improves delayed matching to sample dms performance by monkeys. Federation Proceedings 46(4): 1132

Michelson, Gary Karlin, 2000: Threaded spinal implant. An artificial spinal implant is disclosed which when placed between two adjacent vertebrae directly participates and is incorporated in the ensuing fusion. Instrumentation and procedure is also disclosed.

Makkar H.P.S.; Dawra R.K.; Singh B., 1990: In vitro effect of oak tannins on some hydrolytic and ammonia assimilating enzymes of the bovine rumen. Effect of tannin-containing extract of oak (Quercus incana) leaves on rumen urease, cellulase, protease, amylase, glutmate dehydrogenase (both NADH- and NADPH specific), glutmate-pyruvate transaminase, glutamate oxaloacetate transaminase and gluta...

Alibiglou, L.; Brown, D.A., 2011: Impaired muscle phasing systematically adapts to varied relative angular relationships during locomotion in people poststroke. After stroke, hemiparesis will result in impairments to locomotor control. Specifically, muscle coordination deficits, in the form of inappropriately phased muscle-activity patterns, occur in both the paretic and nonparetic limbs. These dysfunctio...

Coremans, G.; Harlet, L.; Van Cutsem, E.; Vantrappen, G., 1993: Gastric acid suppression increases the risk of Salmonella infection and septicemia. Gastroenterology 104(4 SUPPL ): A686

Hac, Agnieszka Ewa; Seeger, Heiko; Heimburg, Thomas, 2003: Domain formation in planar membranes A fluorescence correlation spectroscopy Study. Biophysical Journal 84(2 Part 2): 326a, February