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Regulation of human T lymphocyte surface antigen mobility by purinergic receptors


, : Regulation of human T lymphocyte surface antigen mobility by purinergic receptors. Journal of Immunology 133(6): 3298-3302

The present study was undertaken to establish whether the capping mechanism of normal human T lymphocytes is regulated by a purinergic receptor. Interaction of T lymphocytes with adenosine significantly increased the mobility of the T3, T4, and T8 surface antigens. This enhanced rate of capping was reflected by a significant decrement in the time intervals to achieve half-maximal capping. T lymphocytes preincubated with theophylline or isobutylmethylxanthine did not exhibit accelerated capping or a decrease in the time required for half-maximal capping in response to adenosine, suggesting that these agents inhibited the binding of adenosine to its receptor. A role for a cAMP-dependent pathway in capping was suggested by the observation that the phosphodiesterase inhibitor RO-201724 caused a decrease in the concentration of adenosine required to accelerate the capping process. Moreover, exposure of T lymphocytes to the cyclic nucleotide derivatives 8-N3-cAMP and 8-Br-cAMP mimicked the effect of adenosine, significantly reducing the time to half-maximal capping. Photoaffinity labeling of intracellular cAMP receptors with 32P-8-N3-cAMP indicated that adenosine caused occupancy of the receptors. This effect of adenosine was inhibited by theophylline, a known purinergic receptor blocker. The data support the concept that the T cell capping mechanism is mediated by an adenylate cyclase-coupled purinergic receptor that activates a cAMP-dependent pathway, and that this pathway is functional in the T3+, T4+, (inducer) and T3+, T8+ (suppressor) subsets.

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Accession: 006288590

PMID: 6092471

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Carr, B. I.; Wang, Z.; Wang, M.; Finn, F. M., 1994: Prothrombin and the vitamin K cycle A novel endogenous hepatic growth inhibitory pathway. Hepatology 20(4 PART 2): 272A

Nelson N.M., 1973: Biochemical lesions in respiratory distress syndrome. Villee, Claude A , Dorothy B Villee And James Zuckerman (Ed ) Respiratory Distress Syndrome Proceedings Of A Conference Dedham, Mass , Usa, May 4-6, Xiii+406p Illus Academic Press: New York, N Y , Usa; London, England 129-146

Yamaguchi, Yumi; Gojobori, Takashi, 1996: Molecular evolution of viruses. Virus (Nagoya) 46(1): 1-6

Ito M., 1988: Studies of pupillary abnormalities in spinocerebellar degeneration. Various pupillary abnormalities are known to be associated with spinocerebellar degeneration (SCD). In this study, infrared oculography was performed in 80 patients with SCD including 8 with Shy-Drager syndrome (SDS), 30 with olivopontocerebellar...

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