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Regulation of hydrocortisone binding sites by hydrocortisone in human bone marrow fibroblasts


, : Regulation of hydrocortisone binding sites by hydrocortisone in human bone marrow fibroblasts. International Journal of Cell Cloning 4(1): 43-50

The incubation of human bone marrow fibroblasts with hydrocortisone (10(-7) M) produces a time-dependent depletion of hydrocortisone-binding sites. This effect is glucocorticoid-specific, since glucocorticoid agonists cause depletion to the same extent as hydrocortisone. After withdrawal of the hormone from the incubation medium, cells are able to replenish their complement of receptor. The existence of this down-regulation process may represent a protective mechanism against the partial proliferative inhibitory action of glucocorticoids for bone marrow fibroblasts and also a stimulus for differentiation to adipocytes.

Accession: 006288600

PMID: 2419458

DOI: 10.1002/stem.5530040105

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Related references

Minguell J.J.; Martinez J.; Walter T., 1982: Effect of hydrocortisone on the growth of human bone marrow fibroblasts. Fibroblast-like cells may be grown from human bone marrow. Adherent cultures show a logarithmic phase of growth lasting 6 days. Hydrocortisone (1 .times. 10-8 M) decreases cell growth in 40-60% when measured as cell number and DNA synthesis. Fibro...

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