EurekaMag.com logo
+ Translate

Regulation of hydrogen per oxide in eye humors effect of 3 amino 1h 1 2 4 triazole on catalase ec 1.11.1.6 and glutathione peroxidase ec 1.11.1.9 of rabbit eye


, : Regulation of hydrogen per oxide in eye humors effect of 3 amino 1h 1 2 4 triazole on catalase ec 1.11.1.6 and glutathione peroxidase ec 1.11.1.9 of rabbit eye. Biochimica et Biophysica Acta 497(3): 641-651

Activities of catalase (H2O2:H2O2 oxidoreductase, EC 1.11.1.6) and GSH [glutathione] peroxidase (GSH:H2O2 oxidoreductase, EC 1.11.1.9) were measured in iris, ciliary body, retina, corneal epithelium, corneal endothelium, lens capsule-epithelium and decapsulated lens. 3-Amino-1H-1,2,4-triazole is a specific inhibitor of catalase and a potent cataractogenic agent. There was marked inhibition of catalase activity in these tissues 1-6 h after the administration of a single i.v. dose of 1 g 3-aminotriazole/kg body weight in rabbit. This was associated with a 2-3-fold increase in the H2O2 concentrations of aqueous humor and vitreous humor. The increased peroxide concentrations were restored to the physiological levels as the catalase activity of eye tissues gradually returned to normal with time after injection. Under the conditions, GSH peroxidase activity of the aforementioned eye tissues was unaltered, GSH and protein sulfhydryl of lens were not changed, and ascorbic acid of aqueous humor and vitreous humor was not significantly altered. Catalase of eye tissues apparently regulates the endogenous H2O2 in eye humors to the physiological level. H2O2 may be the triggering factor in cataract induced by 3-aminotriazole.

(PDF 0-2 workdays service)

Accession: 006288603

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Bhuyan D.K.; Bhuyan K.C., 1975: Regulation of hydrogen per oxide levels in eye humors effect of amino triazole on catalase and glutathione peroxidase activity in rabbit eye tissues. IRCS (International Research Communications System) Medical Science Library Compendium 3(8): 415

Bhuyan, K.C.; Bhuyan, D.K., 1977: Regulation of hydrogen peroxide in eye humors. Effect of 3-amino-1H-1,2,4-triazole on catalase and glutathione peroxidase of rabbit eye. Activities of catalase (H2O2: H2O2 oxidoreductase, EC 1.11.1.6) and GSH peroxidase (GSH: H202 oxidoreductase, EC 1.11.1.9) have been measured in iris, ciliary body, retina, corneal epithelium, corneal endothelium, lens capsule-epithelium and decap...

Cohen, G.; Somerson, N.L., 1969: Enz catalase amino triazole method for measuring secretion of hydrogen per oxide by microorganisms mycoplasma pneumoniae enz peroxidase. Journal of Bacteriology 98(2): 543-546

Anonymous, 1979: Mechanism of cataractogenesis induced by 3 amino 1h 1 2 4 triazole 1 morphology and histo pathology of cataract and the role of catalase in the regulation of hydrogen per oxide in the eye

Jones D.P.; Eklow L.; Thor H.; Orrenius S., 1981: Metabolism of hydrogen per oxide in isolated hepatocytes relative contributions of catalase and glutathione peroxidase in decomposition of endogenously generated hydrogen per oxide. The compartmentation of H2O2 catabolism was studied in isolated hepatocytes. H2O2 generation in the peroxisomal compartment was stimulated by addition of glycolate and in the endoplasmic reticular compartment (cytosolic compartment) by ethylmorphi...

Yi, K.A.; Hong, Y.N.m; Jin, C.D.ck, 1997: Regulation of ascorbate peroxidase activity in dark-grown radish cotyledons by a catalase inhibitor, 3-amino-1,2,4-triazole. The present study was performed to see the physiological role of cytosolic ascorbate peroxidase (APX) and its relationship to other enzymes involved in the H2O2 scavenging metabolism, and also to elucidate the regulation of APX expression in dark-...

Margoliash, E.; Schejter, A., 1962: Kinetics of the irreversible inhibition of catalase by 3-amino-1,2,4-triazole in the presence of hydrogen peroxide and catalase-hydrogen peroxide complex I hydrogen donors. The inhibition of catalase by amino-triazole in the presence of H2O2 is due to an irreversible reaction of the inhibitor with catalase-hydrogen peroxide complex I (Complex I)(Biochem. J., 74 , 339, 1960). In the presence of a Complex I hydrogen do...

Hochstein P.; Utley H., 1968: Hydrogen per oxide detoxication by enz glutathione peroxidase and enz catalase in rat liver homogenates anemia erythrocyte enz glucose 6 phosphate dehydrogenase. Molecular Pharmacology 4(6): 574-579

Valdivia, E.; Martinez, J.; Ortega, J.M.; Montoya, E., 1983: Effect of 3 amino 1 2 4 triazole on the catalase activity of saccharomyces cerevisiae a new procedure for cytochrome c peroxidase ec 1.11.1.5 assay. Yeast cytochrome c peroxidase assay presents 2 different problems, namely electron donor oxidation by cytochrome c oxidase, and rapid decomposition of the substrate (H2O2). A new procedure was described for avoiding these difficulties by using o-d...

Margoliash, E.; Schejter, A., 1962: Kinetics of the irreversible inhibition of catalase by 3-amino-l,2,4-triazole in the presence of hydrogen peroxide and catalase-hydrogen peroxide complex I hydgrogen donors. The inhibition of catalase by amino-triazole in the presence of H2O2 is due to an irreversible reaction of the inhibitor with catalase-hydrogen peroxide complex I (Complex I)(Biochem. J., 74 , 339, 1960). In the presence of a Complex I hydrogen do...