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Regulation of hydrogenase activity in vegetative cells of Anabaena variabilis


, : Regulation of hydrogenase activity in vegetative cells of Anabaena variabilis. Journal of Bacteriology 155(1): 129-137

Heterocyst-free (NH4+-grown) cultures of the cyanobacterium Anabaena variabilis produce a hydrogenase which is reversibly inhibited by light and O2. White or red light at an intensity of 5,000 lx inhibited greater than 95% of the activity. Oxygen at concentrations as low as 0.5% inhibited more than 85% of the hydrogenase in the vegetative cells of CO2-NH4+-grown cultures. The vegatative cell hydrogenase is also sensitive to strong oxidants like ferricyanide. In the presence of strong reductants like S2O4(2-), hydrogenase activity was not inhibited by light. However, hydrogenase activity in the heterocysts was insensitive to both light (greater than 5,000 lx) and O2 (10%). Heterocysts and light-insensitive hydrogenase activity appear simultaneously during differentiation of the vegetative cells into heterocysts (an NH4+-grown culture transferred to NH4+-free, N2-containing medium). This light-insensitive hydrogenase activity was detected several hours before the induction of nitrogenase activity. These results suggest a mode of regulation of hydrogenase in the vegetative cells of A. variabilis that is similar to "redox control" of hydrogenase and other "anaerobic" proteins in enteric bacteria like Escherichia coli.

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Accession: 006288606

PMID: 6408057

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Related references

Spiller, H.B.okjans, G1; Shanmugam, K., 1983: Regulation of hydrogenase activity in vegetative cells of Anabaena variabilis Cyanobacteria, algae. Journal of bacteriology 155(1): 129-137

Rai, A.N.; Rowell, P.; Stewart, W.D.P., 1982: Glutamate synthase ec 1.4.7.1 activity of heterocysts and vegetative cells of the cyanobacterium anabaena variabilis. Glutamate synthase (GOGAT) activities in cell-free extracts of isolated heterocysts and whole filaments of the cyanobacterium A. variabilis Kutz. (ATCC 29413) were determined by measuring [14C]glutamate production from 2-oxo[14C]glutarate or from...

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Serebriakova, L.; Zorin, N., A.; Lindblad, P., 1994: Reversible hydrogenase in Anabaena variabilis ATCC 29413: Presence and localization in non-N-2-fixing cells. The presence and localization of a reversible hydrogenase in non-N-2-fixing cells of the filamentous cyanobacterium Anabaena variabilis were investigated by in vitro activity measurements, native-PAGE/activity stain, SDS-PAGE/Western immunoblots,...

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