+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of hypoxanthine DNA glycosylase in normal human and Bloom's syndrome fibroblasts

, : Regulation of hypoxanthine DNA glycosylase in normal human and Bloom's syndrome fibroblasts. Cancer Research 46(8): 3756-3761

The regulation of the base excision repair enzyme hypoxanthine DNA glycosylase was examined in normal human skin fibroblasts (NHS) and fibroblasts from a patient with Bloom's syndrome. Using randomly proliferating cells and those synchronized at specific intervals in the cell cycle, enzyme levels were shown to become elevated severalfold in a proliferation-associated manner. In NHS synchronized in G0 by serum deprivation or in G1 by isoleucine deprivation, maximal enzyme levels were reached prior to maximal rates of DNA synthesis. In Bloom's syndrome cells synchronized in this manner, these two activities were coincident. Cells synchronized at the G1-S border by hydroxyurea exhibit an initial wave of DNA synthesis upon removal of the drug. The cells then undergo another DNA synthetic cycle climaxing 18-21 h after release. Maximal hypoxanthine glycosylase activity of hydroxyurea-synchronized Bloom's cells was observed during the second round of DNA synthesis. However, in NHS the peak of enzyme activity was observed as early as 9 h prior to the second round of DNA synthesis. To determine if hypoxanthine glycosylase could be induced in the absence of DNA synthesis, serum-synchronized NHS were released in the presence of hydroxyurea. The results showed that inhibition of DNA synthesis did not diminish glycosylase induction which demonstrated that DNA replication was not required for glycosylase induction.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288622

PMID: 3731054

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Colonge, R.; Rafey, A., 1947: Histological changes in the endocrine glands and genital tract of rats completely deprived of vitamin B2. Two rats which had received a normal diet and two deprived of riboflavin were killed when the latter began to lose weight severely. The deprived animals, a male and a female, both showed complete disappearance of the thymus and failure of developm...

Setty, G. Radhakrishnaiah; Vijayalakshmi, M. R.; Devi, A. Usha, 1993: Methods for peeling fruits and vegetables A critical evaluation. Journal of Food Science & Technology 30(3): 155-162

Morris W.G., 1970: Effects of slash burning in over mature stands of the douglas fir g region. Forest Science 16(3): 258-270

Cheng, Y.; Zhong, N., 2003: Interleukin-10 inhibits allergen specific Th2 cytokine expression in allergic asthma. Journal of Allergy & Clinical Immunology 111(2 Abstract Supplement): S118, February

Byrne J.E.; Dresel P.E., 1970: The efficacy of anti arrhythmic drugs in the prevention of recurrence of aconitine induced atrial fibrillation after electrical conversion. Canadian Journal of Physiology & Pharmacology 48(2): 90-97

Blouin, J-Sébastien.; Descarreaux, M.; Bélanger-Gravel, A.; Simoneau, M.; Teasdale, N., 2003: Attenuation of human neck muscle activity following repeated imposed trunk-forward linear acceleration. It has been suggested that, after a passive linear acceleration of a seated subject which resembles a small, rear-end car impact, sensory information from proprioceptive, vestibular, and visual systems elicit stabilizing neck muscular responses. T...

Ohnaka Mitiyasu, 1996: Time scale from nucleation to unstable, fast-speed shear rupture; implications of geometric irregularities of the rupturing surfaces. Eos, Transactions, American Geophysical Union 77(46, Suppl

Jibson Randall W., 1994: Using landslides for paleoseismic analysis. Open-File Report - U S

Zha, H-Guang.; Liu, T.; Zhou, J-Jiang.; Sun, H., 2014: MS-desi, a desiccation-related protein in the floral nectar of the evergreen velvet bean (Mucuna sempervirens Hemsl): molecular identification and characterization. Plant desiccation-related proteins (DRPs) were first identified as pcC13-62 from the resurrection plant <em>Craterostigma plantagineum</em> and it has been suggested they are involved in plant desiccation tolerance. We identified and c...

Heuze, J. P.; Fontayne, P., 2002: Questionnaire sur l'Ambiance du Groupe: a French-language instrument for measuring group cohesion. The present report provides a summary of 5 studies undertaken to develop a French-language instrument to assess cohesiveness in sport teams - the "Questionnaire sur l'Ambiance du Groupe" (QAG). For the initial version of the instrum...