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Regulation of idiotope expression 1. the effect of antigen dose on expression of certain t 15 idiotopes during primary immunoglobulin m response to streptococcus pneumoniae r 36a


, : Regulation of idiotope expression 1. the effect of antigen dose on expression of certain t 15 idiotopes during primary immunoglobulin m response to streptococcus pneumoniae r 36a. Journal of Immunology 134(3): 1926-1929

Clonal heterogeneity among mouse B cells reactive to the same epitope may be determined through differences in idiotypy. It appears that clones bearing distinct idiotopes may constitute functionally distinct subpopulations. Data suggest that idiotopically distinct clones of PC [phosphocholine]-reactive B cells may be regulated independently of one another. It was determined whether individual T15+ clones may also differ in their requirements for activation. The effect of immunizing doses of antigen on expression of 2 T15 idiotopes, B36-82 and B39-38, during both in vivo and in vitro primary responses to S. pneumoniae R36a (Pn) was examined in CB-20 mouse strain. The idiotopes were detected on the specific antibody plaque-forming cells (PFC) by using monoclonal anti-idiotopic antibodies. Distinct patterns of idiotope expression are generated by stimulation with different doses of antigen. Immunization with suboptimal and superoptimal doses of Pn produced responses dominated by PFC expressing both idiotopes. PFC induced by optimal antigen concentrations were primarily B36-82+ and B39-38-. These data indicate that the varying of antigen concentration may induce the response of different B cells bearing distinct idiotypes.

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Related references

Stout, J.T.; Strickland, F.M.; Cerny, J., 1985: Regulation of idiotope expression. I. The effect of antigen dose on expression of certain T15 idiotopes during primary IgM response to S. pneumoniae R36a. Clonal heterogeneity among B cells reactive to the same epitope may be determined through differences in idiotypy. It appears that clones bearing distinct idiotopes may constitute functionally distinct subpopulations. Data suggest that idiotopical...

Cronkhite, R.; Schulze, D.; Cerny, J., 1989: Regulation of idiotope expression. IV. Genetic linkage of two D region-dependent T15 idiotopes to the IgH allotype. The idiotopic (Id) repertoire of antibody response to phosphocholine was studied in mouse strains with different IgH allotypes. The T15 idiotype-bearing (T15+) serum antibody and antibody plaque-forming cells (PFC) were characterized with four mon...

Cerny, J.; Heusser, C.; Wallich, R.; Hammerling, G.J.; Eardley, D.D., 1982: Immunoglobulin idiotopes expressed by T cells. I. Expression of distinct idiotopes detected by monoclonal antibodies on antigen-specific suppressor T cells. The idiotopic repertoire expressed by antigen-specific suppressor T cells (Ts) generated by Streptococcus pneumoniae strain R36a (Pn) in BALB/c strain mice was investigated using a panel of five monoclonal anti-idiotopic antibodies against TEPC-15...

Hendriksen, W.T.; Silva, N.; Bootsma, H.J.; Blue, C.E.; Paterson, G.K.; Kerr, A.R.; de Jong, A.; Kuipers, O.P.; Hermans, P.W.M.; Mitchell, T.J., 2006: Regulation of gene expression in Streptococcus pneumoniae by response regulator 09 is strain dependent. Recent murine studies have demonstrated that the role of response regulator 09 (RR09) of Streptococcus pneumoniae in virulence is different in different strains. In the present study, we used a murine pneumonia model of infection to assess the vi...

Legrain, P.; Buttin, G., 1983: The level of expression and the molecular distribution of ABPC 48 idiotopes in levan- or anti-idiotope-primed BALB/c mice. Annals of the New York Academy of Sciences 418: 290-295

Cerny J., 1986: Factors influencing the expression of t 15 idiotopes during an antigen driven response to phosphorylcholine. Federation Proceedings: 730

Kelsoe, G.; Reth, M.; Rajewsky, K., 1981: Control of idiotope expression by monoclonal anti-idiotope and idiotope-bearing antibody. Preinjection of C57BL/6 mice with nano-to microgram amounts of a monoclonal IgG1 antibody directed against a binding site-related idiotope of the anti-NP [(4-hydroxy-3-nitro-phenyl)acetyl] antibody B1-8 results in enhancement or suppression of the...

Berberich, I.; Schimpl, A., 1992: Regulation of immunoglobulin gene expression in normal lymphocytes. II. Mechanisms of down-regulation of immunoglobulin secretion after engagement of the B cell antigen receptor. When B cells are stimulated with lipopolysaccharide (LPS) they start to proliferate and mature into immunoglobulin (Ig)-secreting cells. Co-stimulation with F(ab')2 fragments of antibodies directed against the B cell antigen receptor leads to...

Thornton, B.P.; Vetvicka, V.; Ross, G.D., 1996: Function of C3 in a humoral response: iC3b/C3dg bound to an immune complex generated with natural antibody and a primary antigen promotes antigen uptake and the expression of co-stimulatory molecules by all B cells, but only stimulates immunoglobulin synthesis by antigen-specific B cells. Previous studies have shown that an optimal humoral response to a primary protein antigen requires C3 and CR2 (CD21). Sera from non-immunized donors contain natural IgM and IgG antibodies to the primary antigen keyhole limpet haemocyanin (KLH), an...

Thornton, B.P.; Vetvicka, V.; Ross, G.D., 1996: Function of C3 in a humoral response: iC3b/C3dg bound to an immune complex generated with natural antibody and a primary antigen promotes antigen uptake and the expression of co-stimulatory molecules by all B cells, but only stimulates immunoglobulin synthesis by antigen-specific B cells. Previous studies have shown that an optimal humoral response to a primary protein antigen requires C3 and CR2 (CD21). Sera from non-immunized donors contain natural IgM and IgG antibodies to the primary antigen keyhole limpet haemocyanin (KLH), an...