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Regulation of iga differentiation in ch12lx b cells by lymphokines il 4 induces membrane igm positive ch12lx cells to express membrane iga and il 5 induces membrane iga positive ch12lx cells to secrete iga


, : Regulation of iga differentiation in ch12lx b cells by lymphokines il 4 induces membrane igm positive ch12lx cells to express membrane iga and il 5 induces membrane iga positive ch12lx cells to secrete iga. Journal of Immunology 141(3): 713-720

In these studies we utilized the Ag(SRBC)-reactive B cell line CH12LX to study isotype switching. CH12LX cells are a stable population of B cells mainly bearing membrane IgM (mIgM) (98 to 99%) with a small population of B cells bearing membrane IgA (mIgA) (1 to 2%). LPS induced a 5- to 10-fold increase in the secretion of both Ig, whereas a lymphokine-rich supernatant of D10 T cells induced a greater increase in the secretion of IgA than IgM. Analysis of the latter effect with recombinant lymphokines disclosed that rIL-4 induced an increase in the number of mIgA+ cells (6 to 15%) with minimal effect on IgA secretion, whereas IL-5 induced increased IgA secretion but had no effect on mIgA expression. The addition of both lymphokines induced increased mIgA expression and IgA secretion. No effect on mIgA expression or IgA secretion was seen with other lymphokines, including IL-1, IL-2, IL-3, IL-6, GM-CSF, and IFN-.gamma. The rIL-4 effect on CH12LX cells represents true differentiation rather than selective proliferation for the following reasons: first, subclones of CH12LX cells respond to IL-4-containing T cell supernatant in the same fashion as the original cell line; second, culture of CH12LX cells wit IL-4 causes the appearance of large numbers of dual-bearing mIgM/mIgA cells as well as mIgA+ cells and a dual-bearing mIgM/mIgA line was obtained by cloning CH12LX after stimulation with an IL-4-containing supernatant; third, sorted mIgA+ and mIgA- CH12LX cells had similar rates of proliferation in the presence or absence of IL-4. In further studies, it was found that IL-5 causes IgA secretion by mIgA+ but not mIgA- Ch12LX cells indicating that it is acting as a post-isotype switch differentiation factor. These studies are consistent with the view that IL-4 and IL-5 act in a sequential fashion to induce IgA expression and secretion in CH12LX cells, IL-4 inducing differentiation of mIgM+ cells to mIgA+ cells and IL-5 enhancing the IgA secretion by the resulting mIgA-bearing cells.

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Henry Gautier, 1973: Respiratory responses of the anesthetized rabbit to vagotomy and thoracic dorsal rhizotomy. Respiration Physiology 17(2): 238-247

Grout, A. J., 1926: Musci Acrocarpi Boreali-Americani et Europaei. The 24th fascicle of the series is being distributed by John M. Holzinger. Nine of the 25 specimens included are European. Two new American species with descriptions on labels are reported. These are Fissidens manateensis A. J. Grout, Florida, and...

Baumann, R.; Haller, E. A.; Padeken, S., 1981: Oxygen binding properties of embryonic chicken blood during early development. Pflugers Archiv 389(Suppl.): R50

Eldridge, Edward F., 1963: Irrigation as a source of water pollution. Trends in extent of irrigation of farmlands are reviewed and estimates are presented of return irrigation flows to surface and ground waters. Quality changes resulting from such return flows are discussed, including specific citations of salinity,...

Wilcox, J. R-Jr ; Moniot, A. L.; Green, J. P., 1977: Bone scanning in the evaluation of exercise related stress injuries. Bone scintigraphy was used to evaluate 34 patients with the physical findings and history of stress fracture of the lower extremity. Of the 34, 21 had abnormal studies, 11 involving the femoral neck, 9 the tibia and 1 the femur. All of these had a...

Lamey, Ha, 1970: Collection of viruliferous plant hoppers with a compressed air aspirator. Plant disease reporter 54 (1) 56-58

Blakeley A.G.H.; Brown G.L.; Dearnaley D.P.; Woods R.I., 1968: The use of prostaglandin e 1 hematol in perfusion of the spleen with blood abstract cat reduction of platelet aggregation. Journal of Physiology (Cambridge) 198(1): 31-32

Templer, J. C., 1978: Tea branch lesions and deaths. Failure to recover from pruning, external damage (sun-scorch, frost damage and mechanical damage) and attacks by disease-causing organisms are commonly occurring disorders on tea branches in East Africa. Research on these problems is briefly discu...

Moscato G.; Dellabianca A.; Bertoletti R.; Marraccini P.; Vinci G.; Gherson G., 1992: Bronchial responsiveness to ultrasonic fog in occupational asthma due to toluene diisocyanate tdi. American Review of Respiratory Disease 145(4 PART 2): A322

Kil, S. J.; Hobert, M. E.; Carlin, C. R., 1997: A dileucine motif in the EGF receptor juxtamembrane domain is critical for post-endocytic trafficking to lysosomes. Molecular Biology of the Cell 8(SUPPL ): 422A, Nov