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Regulation of immune responses by i j gene products 1. production and characterization of anti i j mono clonal antibodies

, : Regulation of immune responses by i j gene products 1. production and characterization of anti i j mono clonal antibodies. Journal of Experimental Medicine 154(5): 1570-1583

Using a novel, 2-step functional screening procedure, hybridoma B cell lines secreting monoclonal antibodies directed against gene products of the I-Jb and I-Jk subregions of the mouse H-2 complex were isolated. These monoclonal antibodies act in vitro by allowing nonresponder spleen cells to respond to normally suppressive quantities of poly(Glu50Tyr50) (GT) (WF8 series of anti-I-Jk monoclonal antibodies) or to suboptimal concentration of poly(Glu60Ala30Tyr10) (WF9 series of anti-I-Jb monoclonal antibodies). Some of the culture supernates that show augmenting activity bind GT-specific T cell-derived suppressor factor (GT-TsF), indicating that some monoclonal antibodies display a nonspecific enhancing effect, or, more likely, that anti-I-J monoclonal antibodies have been produced against I-J determinants not found on TsF. This last possibility is most intriguing and might serve as a means for exploring the heterogeneity of the I-J subregion. Some of these monoclonal anti-I-J antibodies might detect antigenic determinants selectively expressed on suppressor T cells, helper T cells and/or macrophages. Monoclonal anti-I-J antibodies evidently should be useful in the biochemical characterization and purification of a monoclonal GT-TsF. These haplotype-specific anti-I-J monoclonal antibodies should prove to be powerful tools for future studies exploring the role of I-J gene products in the regulation of specific immune responses.

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