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Regulation of immunoglobulin g memory responses by helper and suppressor t cells activated by the type 2 antigen polyvinylpyrrolidone


, : Regulation of immunoglobulin g memory responses by helper and suppressor t cells activated by the type 2 antigen polyvinylpyrrolidone. Journal of Experimental Medicine 161(6): 1357-1367

T cells from CAF1 mice immunized with various amounts of the type 2 antigen polyvinylpyrrolidone (PVP) were assessed for their ability to provide help to PVP-specific memory B cells for the production of IgG. Low doses (0.0025 .mu.g) of PVP consistently activated helper T cells (Th), which were required for the production of IgG by primed B cells. T cells from mice primed with higher amounts (0.25 or 25 .mu.g) of PVP did not provide significant help to the same B cells for IgG production. When mixed with B cells and low-dose PVP-primed Th, T cells from mice primed with 0.25 or 25 .mu.g PVP suppressed PVP-specific IgG, but not IgM antibody responses. The suppressor cells induced by higher amounts of PVP were eliminated either by injecting cyclophosphamide (CY) before priming with PVP, or by treating the primed T cells with anti-Lyt-2.2 and complement before transfer. Pretreatment of suppressor T cell (Ts) donors with CY or removal of Lyt-2+ T cells not only eliminated Ts activity, but also unmasked significant Th activity in the T cells from high-dose PVP-primed mice. Thus, both low and high amounts of PVP can activate Th, although high amounts of PVP also induce Ts, the activity of which predominates in a normal unfractionated T cell population. The amount of PVP (0.0025 .mu.g) that induces dominant help for IgG memory responses was only marginally immunogenic for induction of primary PVP-specific IgM responses, while 0.25 and 25 .mu.g PVP, which induce dominant suppression for IgG memory responses, are optimally immunogenic for primary IgM responses. These results are discussed in the context of the inability of most type 2 antigens to elicit primary IgG responses or to prime memory B cells for production of IgG, responses which are dependent on the function of antigen-specific Th.

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Related references

Braley-Mullen, H., 1985: Regulation of IgG memory responses by helper and suppressor T cells activated by the type 2 antigen, polyvinylpyrrolidone. T cells from CAF1 mice immunized with various amounts of the type 2 antigen polyvinylpyrrolidone (PVP) were assessed for their ability to provide help to PVP-specific memory B cells for the production of IgG. Low doses (0.0025 micrograms) of PVP c...

Vanbuskirk A.; Braley Mullen H., 1985: Regulation of immunoglobulin g memory responses by helper t cells activated by polyvinylpyrrolidone. Federation Proceedings 44(5): 1715

Van Buskirk, A.M.; Braley-Mullen, H., 1987: Suppression of IgG memory responses by T cells activated with the type 2 antigen polyvinylpyrrolidone (PVP). Although type 2 antigens, such as polyvinylpyrrolidone (PVP), generally do not prime for IgG memory responses or activate specific helper T cells (TH), previous studies have established that low doses of PVP (0.0025 microgram) can prime for IgG me...

Van Buskirk, A.M.; Braley-Mullen, H., 1987: In vitro activation of specific helper and suppressor T cells by the type 2 antigen polyvinylpyrrolidone (PVP). Although type 2 antigens, such as PVP, generally do not activate specific TH, previous studies have established that low doses of PVP (0.0025 microgram) can activate TH in vivo which provide help in primed B cells for PVP-specific IgG responses. D...

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