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Regulation of immunoglobulin production after human marrow grafting the role of helper and suppressor t cells in acute graft vs. host disease






Transplantation (Baltimore) 41(3): 328-335

Regulation of immunoglobulin production after human marrow grafting the role of helper and suppressor t cells in acute graft vs. host disease

The effect of acute graft-versus host disease (GVHD) on T4 and T8 lymphocyte regulation of in vitro immunoglobin production was explored. The peripheral blood lymphocytes from 20 patients were studied sequentially in the first 100 days after sibling bone marrow grafting for hematologic malignancy or aplastic anemia. T and non-T lymphocytes were prepared from peripheral blood by Ficoll-Hypaque density gradient centrifugation and sheep erythrocyte rosetting. T cells were enriched for T4 or T8 cells and cocultured for six days with pokeweed mitogen and autologous non-T or T and non-T cells from unrelated normal individuals. Immunoglobulin production was assessed using a reverse hemolytic plaque assay. All three patients without acute GVHD had failure of non-T cells to secrete immunoglobulin, one had failure of helper T cell activity, and 2 developed suppressor T cells. Similarly, all six patients studied sequentially after the development of GVHD had non-T-cell failure, five developed helper T cell failure, and five had suppressor T cells. These data suggest no difference in lymphocyte function before or after the development of acute GVHD. When the T cells of these patients were split into T4 and T8 subpopulations and studied for immunoglobulin production there was helper T cell failure in 4 of 9 tests with enriched T4 populations. Five of 9 tests with T8 enriched populations showed suppressor activity. Suppressor T cell function was also seen in 4 of 9 tests with T4-enriched populations. These data show that T cell function does not necessarily correlate with the surface phenotype during the first 100 days after grafting. A role for cytomegalovirus (CMV) infection in bringing out suppressor activity is suggested, because among patients without GVHD, 6 of 8 tests in CMV-positive patients showed suppressor cells compared with none of 4 tests in patients without CMV infection.


Accession: 006288694



Related references

Witherspoon, R.P.; Goehle, S.; Kretschmer, M.; Storb, R., 1986: Regulation of immunoglobulin production after human marrow grafting. The role of helper and suppressor T cells in acute graft-versus-host disease. The effect of acute graft-versus host disease (GVHD) on T4 and T8 lymphocyte regulation of in vitro immunoglobulin production was explored. The peripheral blood lymphocytes from 20 patients were studied sequentially in the first 100 days after sib...

Rolink, A.G.; Radaszkiewicz, T.; Pals, S.T.; Van-Der-Meer, W.G.J.; Gleichmann, E., 1982: Allo suppressor and allo helper t cells in acute and chronic graft vs. host disease 1. allo reactive suppressor cells rather than killer t cells appear to be the decisive effector cells in lethal graft vs. host disease. Splenic T cells from B10 donors were injected into irradiated (B10 .times. DBA/2)F1 mice. Either 5 or 6 days later, activated donor T cells were recovered from the spleens of these primary F1 (1.degree. F1) recipients and transferred to groups of...

Pals, S.T.; Radaszkiewicz, T.; Gleichmann, E., 1984: Allo suppressor t cell and allo helper t cells in acute and chronic graft vs. host disease 4. activation of donor allo suppressor cells is confined to acute graft vs. host disease. Groups of nonirradiated BDF1 mice were injected with unseparated spleen cells from B10, B10.D2 or DBA/2 donors. The diverse clinical and pathologic symptoms that developed during the course of the ensuing graft-vs.-host reaction (GVHR) were relate...

Pals, S.T.; Gleichmann, H.; Gleichmann, E., 1984: Allo suppressor and allo helper t cells in acute and chronic graft vs. host disease 5. f 1 mice with secondary chronic graft vs. host disease contain f 1 reactive allo helper but no allo suppressor t cells. The alloreactive properties of donor T cells obtained from F1 mice that had recovered from the allosuppression of acute graft vs. host disease (GVHD) and showed mild symptoms of chronic GVHD, i.e., so-called secondary chronic GVHD, were studied. (...

Tsoi, M.S.; Storb, R.; Dobbs, S.; Kopecky, K.J.; Santos, E.; Weiden, P.L.; Thomas, E.D., 1979: Nonspecific suppressor cells in patients with chronic graft-vs-host disease after marrow grafting. Forty-four human long-term survivors after marrow transplantation for aplastic anemia or hematologic malignancy were studied for the presence of circulating nonspecific suppressor cells. Twenty-two of the patients were healthy and 22 had mild to m...

Anonymous, 1981: Specific suppressor cells and immune response to host antigens in long term human allogeneic marrow recipients implications for the mechanisms of graft host tolerance and chronic graft vs host disease

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Atkinson, K.; Farewell, V.; Storb, R.; Tsoi, M.S.; Sullivan, K.M.; Witherspoon, R.P.; Fefer, A.; Clift, R.; Goodell, B.; Thomas, E.D., 1982: Analysis of late infections after human bone marrow transplantation: role of genotypic nonidentity between marrow donor and recipient and of nonspecific suppressor cells in patients with chronic graft-versus-host disease. Infections occurring 6 mo or later after bone marrow transplantation for severe aplastic anemia or hematologic malignancy were analyzed in 98 long-term survivors. Varicella-zoster (VZ) infections were analyzed separately from all other infections....

Witherspoon, R.P.; Matthews, D.; Storb, R.; Atkinson, K.; Cheever, M.; Deeg, H.J.; Doney, K.; Kalbfleisch, J.; Noel, D.; Et-Al, 1984: Recovery of in vivo cellular immunity after human marrow grafting influence of time post grafting and acute graft vs. host disease. Marrow graft recipients (332) and 241 healthy marrow donors were studied by skin testing with recall and neoantigens. Patients (230) with leukemia and 78 patients with aplastic anemia received allogeneic HLA-identical sibling marrow. Patients (24)...

Rolink, A.G.; Gleichmann, E., 1983: Allo suppressor t cells and allo helper t cells in acute and chronic graft vs. host disease 3. different lyt subsets of donor cells induce different pathological syndromes. The Lyt phenotypes of B10 donor T cells required for the induction of acute or chronic GVHD [graft vs. host disease] in H-2-different (B10 .times. DBA/2)F1 recipients were analyzed. Nonirradiated F1 mice were used as the recipients. Unseparated B1...