EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of immunoglobulin synthesis by human t cell subsets as defined by anti d 44 monoclonal antibody within the cd 4 positive and cd 8 subpopulations


Journal of Immunology 136(4): 1144-1149
Regulation of immunoglobulin synthesis by human t cell subsets as defined by anti d 44 monoclonal antibody within the cd 4 positive and cd 8 subpopulations
In our previous paper, we demonstrated that anti-D44 MAb can, in the presence of complement, eliminate all the allocytotoxicity generated during a mixed lymphocyte reaction without affecting the alloproliferative response. As approximately 70% of CD4+ cells and 30% of CD8+ will be stained with anti-D44 MAb, we researched the functional role of the D44+ and D44- cells in each of these T cell subsets in the PWM-induced antibody response. We found that most of the helper activity for immunoglobulin (Ig) synthesis was mediated by CD4+ D44+ lymphocytes and that virtually all the suppressive activity was mediated by CD8+ D44- lymphocytes. Surprisingly enough, we noticed that the low level of Ig synthesis induced in B cells by CD4+ D44- lymphocytes could be strongly amplified by the addition of radiosensitive CD8+ lymphocytes, suggesting coexisting opposite immunoregulatory functions within the CD8+ T cell subset. These results, together with previous data, indicate that anti-D44 MAb subdivides T cells into subpopulations with distinct functional repertoires: a CD4+ D44+ helper subpopulation, a CD8+ D44+ cytotoxic subpopulation, and a CD8+ D44- suppressor subpopulation.


Accession: 006288697



Related references

Regulation of immunoglobulin synthesis by human T cell subsets as defined by anti-D44 monoclonal antibody within the CD4+ and CD8+ subpopulations. Journal of Immunology 136(4): 1144-1149, 1986

In vitro regulation of immunoglobulin synthesis by T-cell subpopulations defined by a new human T-cell antigen (9.3). Cellular Immunology 72(1): 122-129, 1982

T cell subpopulations defined by monoclonal antibodies after hla identical sibling marrow transplantation ii. activated and functional subsets of helper inducer and cytotoxic suppressor subpopulations defined by two color fluorescence flow cytometry. Bone Marrow Transplantation 1(2): 121-132, 1986

T cell subpopulations defined by monoclonal antibodies after HLA-identical sibling marrow transplantation. II. Activated and functional subsets of helper-inducer and cytotoxic-suppressor subpopulations defined by two-colour fluorescence flow cytometry. Bone Marrow Transplantation 1(2): 121-132, 1986

Studies on T-cell subsets in atopic dermatitis: human T-cell subpopulations defined by specific monoclonal antibodies. Clinical Immunology and Immunopathology 22(1): 139-146, 1982

Functional analysis of human T cell subsets defined by monoclonal antibodies. III. Regulation of helper factor production by T cell subsets. Journal of Immunology 126(5): 1948-1951, 1981

Induction of immunoglobulin secreting cells in the human autologous mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets defined with monoclonal antibodies. Journal of Immunology 127(5): 2130-2135, 1981

Human T cell subpopulations defined by a monoclonal antibody. II. Evidence for cell cooperation in the response to alloantigens and generation of cytolytic cells. Journal of Immunology 128(1): 20-23, 1982

Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression. Journal of Experimental Medicine 150(6): 1472-1482, 1979

Differential effects of anti cd45 monoclonal antibody on human b cell proliferation a monoclonal antibody recognizing a neuraminidase sensitive epitope of the t200 molecule enhances anti immunoglobulin induced proliferation. European Journal of Immunology 20(12): 2801-2804, 1990