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Regulation of in vitro androgenesis in tobacco nicotiana tabacum cultivar samsun through iron free media


, : Regulation of in vitro androgenesis in tobacco nicotiana tabacum cultivar samsun through iron free media. Biologia Plantarum (Prague) 21(6): 412-417

Globular embryoids were continually produced in anther cultures of (N. tabacum L. cv. Samsun) from the pool of resting microspores if the Fe-free medium was used. The supplement of Fe stopped the development of fresh early embryoids still inducing continual conversion of the resting globular embryoids into torpedo-shaped embryoids, and into haploid plants. Globular embryoids in the anthers responded to the Fe supply even after 8 mo. cultivation on Fe-free media. Isolated embryoids showed the same response. Haploid plants were regenerated from the anthers on the minimal medium consisting of agar, sucrose, Fe and distilled water. The Fe requirements of preglobular, globular and postglobular embryoids are discussed.

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