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Regulation of induced thionein gene expression in cultured mammalian cells effects of protein synthesis inhibition on translatable thionein messenger rna levels in regulatory variants of the cho cell


Biochemical & Biophysical Research Communications 93(2): 343-348
Regulation of induced thionein gene expression in cultured mammalian cells effects of protein synthesis inhibition on translatable thionein messenger rna levels in regulatory variants of the cho cell
The effects of the protein synthesis inhibitors cycloheximide and puromycin on functional thionein mRNA levels were examined to further delineate the regulation of thionein gene expression in Cd2+-resistant CHO [chinese hamster ovary] cell sublines proficient in Cd2+- or Zn2+-stimulated synthesis of metallothioneins. Inhibition of protein synthesis at or shortly before the time of metal-mediated induction only slightly reduces the usual increase in thionein mRNA. Protein synthesis inhibition alone does not significantly increase thionein mRNA levels. Addition of cycloheximide 4 h after Zn2+ induction increases thionein mRNA activity measured 8 h after induction by at least 40%, suggesting that protein synthesis is required for normal turnover of thionein mRNA.


Accession: 006288731



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