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Regulation of induced thionein gene expression in cultured mammalian cells effects of protein synthesis inhibition on translatable thionein messenger rna levels in regulatory variants of the cho cell


, : Regulation of induced thionein gene expression in cultured mammalian cells effects of protein synthesis inhibition on translatable thionein messenger rna levels in regulatory variants of the cho cell. Biochemical & Biophysical Research Communications 93(2): 343-348

The effects of the protein synthesis inhibitors cycloheximide and puromycin on functional thionein mRNA levels were examined to further delineate the regulation of thionein gene expression in Cd2+-resistant CHO [chinese hamster ovary] cell sublines proficient in Cd2+- or Zn2+-stimulated synthesis of metallothioneins. Inhibition of protein synthesis at or shortly before the time of metal-mediated induction only slightly reduces the usual increase in thionein mRNA. Protein synthesis inhibition alone does not significantly increase thionein mRNA levels. Addition of cycloheximide 4 h after Zn2+ induction increases thionein mRNA activity measured 8 h after induction by at least 40%, suggesting that protein synthesis is required for normal turnover of thionein mRNA.

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Enger, M.D.; Rall, L.B.; Walters, R.A.; Hildebrand, C.E., 1980: Regulation of induced thionein gene expression in cultured mammalian cells: effects of protein synthesis inhibition of translatable thionein mRNA levels in regulatory variants of the CHO cell. Biochemical and Biophysical Research Communications 93(2): 343-348

Enger M.D.; Rall L.B.; Walters R.A.; Hildebrand C.E., 1979: Thionein gene expression in cadmium resistant variants of cho cells effects of protein synthesis inhibition on induced messenger rna levels. Journal of Cell Biology 83(2 PART 2): 401A

Enger, M.D.; Rall, L.B.; Hildebrand, C.E., 1979: Thionein gene expression in Cd++-variants of the CHO cell: correlation of thionein synthesis rates with translatable mRNA levels during induction, deinduction, and superinduction. The relationship of thionein synthesis rates to translatable cytoplasmic thionein mRNA levels was investigated for the first time in a cultured cell system. Thionein synthesis was induced in Cdr, a cadmium-resistant variant of CHO, by exposure to...

Hildebrand C.E.; Rall L.B.; Enger M.D., 1979: Induced specific gene expression in cultured cells correlation of cadmium stimulated thionein synthesis rates with messenger rna levels during induction di induction and super induction. Federation Proceedings 38(3 PART 1): 398

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