+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of initiation factors during translational repression caused by serum depletion. Abundance, synthesis, and turnover rates

, : Regulation of initiation factors during translational repression caused by serum depletion. Abundance, synthesis, and turnover rates. Journal of Biological Chemistry 260(9): 5486-5492

During growth in unreplenished medium, the fraction of active, polysomal ribosomes progressively decreases .apprx. 3-fold from 80-90% to only 20-40% due to a reduced rate of initiation. To assess whether the abundance of initiation factors could be involved in this repression of translational activity, HeLa cell cytoplasmic lysates were resolved by 2-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and spots corresponding to the initiation factor proteins were quantitated. The relative abundance of most of the initiation factor proteins only decreases by 10-40% and roughly parallels that of the ribosomes. Measurement of the rates of synthesis and turnover of the initiation factor proteins establishes that during periods of active growth, synthesis and degradation occur coordinately with total cell protein. As growth rate decreases, the synthesis of some initiation factor proteins, particularly eukaryotic initiation factor (eIF)-3 subunits, becomes depressed. Serum stimulation of serum-depleted cells recruits most inactive ribosomes and mRNA into polysomes, but most initiation factor mRNA are not selectively recruited. The principal exceptions are eIF-3p24 which exhibits 4- to 5-fold enhanced synthesis, and eIF-3p44 and eIF-4A whose syntheses are moderately stimulated.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288740

PMID: 3988764

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Sanberg P.R.; Emerich D.F.; Mcdermott P.E.; Frydel B.R.; Bertino A.M.; Kaplan F.A.; Palmatier M.A.; Christenson L., 1991: Transplantation of polymer encapsulated bovine adrenal chromaffin cells prevent quinolinic acid induced lesions of the striatum. Society for Neuroscience Abstracts 17(1-2): 903

Mleczek, Mław.; Siwulski, M.; Stuper-Szablewska, K.; Rissmann, I.; Sobieralski, K.; Goliński, P., 2013: Accumulation of elements by edible mushroom species: part I. Problem of trace element toxicity in mushrooms. The aim of this study was to evaluate Cd, Co, Cu, Hg, Ni, Pb, Sr and Zn accumulation in six edible mushroom species and to assess their risk and benefits to human consumers. Mushrooms (Leccinium aurantiacum, Xerocomus badius, Lactarius deliciosus,...

Anonymous, 1978: Mixer. This mixer consists of an upright cylindrical chamber with a conical base and a screw conveyor. The screw is encased in a cylindrical sleeve formed of a number of tubular sections separated by gaps which provide inlets and outlets for the material...

Subramanian, S.; Balasubramanian, K. M.; Ranganathan, T. B.; Sivasubramanian, V.; Chandramohan, J., 1984: Co 43, a salt-tolerant variety for Tamil Nadu. TNAU17005, a high yielding, semidwarf, lodging resistant rice with fine, white grains has been released as Co43 for general cultivation in Tamil Nadu during navarai (January-May) and samba (August-November). In trials from 1975 to 1981, and under...

Becker, David W.; Brand, Jerry J., 1985: Anacystis nidulans demonstrates a photosystem II cation requirement satisfied only by Ca2+ or Na+1. Plant Physiology 79: 2-8

Hilario E.; Unda F.; Alino S.F., 1988: Differential distribution of b16f10 melanoma cells in the liver lobule with 1 color plate. The distribution pattern and the number of tumor cells arrested in the liver were studied in mouse livers. Mice were perfused intravascularly with a suspension of B16F10 melanoma cells. The animals were sacrificed at 0, 1, 5 and 20 min after tumor...

Zander R.H.; Eckel P.M., 1982: Hymenostylium recurvirostrum var insigne new record and barbula amplexifolia new record for british columbia canada. H. recurvirostrum var. insigne (Dix.) Bartr. is reported new to the New World from British Columbia. H. recurvirostrum var. cylindricum (Bartr.) Zander [H. glaucum var. cylindricum] is a new combination. A key to the varieties of H. recurvirostrum...

Milarski K.L.; Welch W.J.; Morimoto R.I., 1989: Cell cycle dependent association of hsp70 with specific cellular proteins. In asynchronous populations of HeLa cells maintained at control or heat shock temperatures, HSP70 levels and its subcellular distribution exhibit substantial heterogeneity as demonstrated by indirect immunofluorescence with HSP70-specific monoclon...

Von Jarchow, B. L., 1945: Woodpeckers nests. Woodpeckers excavate in trees oblong cavities, the axes of which are parallel to that of the trunk, with entrance at the upper end. The entrance is circular and proportionately sized for the spp.; e.g., of the downy woodpecker the av. is 3.2 cm.;...

Kettunen, J.A.; Harilainen, A.; Sandelin, J.; Schlenzka, D.; Hietaniemi, K.; Seitsalo, S.; Malmivaara, A.; Kujala, U.M., 2007: Knee arthroscopy and exercise versus exercise only for chronic patellofemoral pain syndrome: a randomized controlled trial. Background: Arthroscopy is often used to treat patients with chronic patellofemoral pain syndrome (PFPS). As there is a lack of evidence, we conducted a randomized controlled trial to study the efficacy of arthroscopy in patients with chronic PFP...