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Regulation of inorganic phosphate uptake by acer pseudoplatanus cells

, : Regulation of inorganic phosphate uptake by acer pseudoplatanus cells. Archives of Biochemistry & Biophysics 219(2): 371-378

In view of the importance of Pi in the control of cell metabolism, it was of interest to study the mechanism and regulation of Pi uptake by A. pseudoplatanus cells grown as cell suspensions. At low external Pi concentrations up to 10 mM, sycamore cells incorporate phosphate against a concentration gradient, by a process which is energy dependent. Under these conditions the intracellular Pi concentration is maintained constant (2-3 mM). On the contrary at high external Pi concentrations, higher than that which counterpoises the cytoplasmic Pi concentration (.apprx. 10 mM), Pi enters the cell by slow diffusion and the intracellular Pi concentration increases continuously as the extracellular Pi concentration increases from 15 to 50 mM. When sycamore cells are transferred to a phosphate-deficient medium, growth slows down considerably and ceases after 4-5 days. During this time, intracellular Pi concentration falls from 3 to 0.1 mM and phosphate esters from 8 to 2 mM. Phosphate starvation stimulates the uptake indicating that phosphate uptake depends on the intracellular phosphate and/or cytoplasmic ester-P pool. Pi uptake by Pi-starved cells is strongly dependent on the pH of the medium.

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