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Regulation of insulin and insulin like growth factor responsiveness by insulin like growth factors in rat hepatoma cells

, : Regulation of insulin and insulin like growth factor responsiveness by insulin like growth factors in rat hepatoma cells. Endocrinology 118(6): 2555-2560

We have previously reported that insulin induces a complete and reversible desensitization to the induction of tyrosine aminotransferase by insulin and insulin-like growth factors (IGFs) in HTC rat hepatoma cells. This loss of responsiveness cannot be accounted for by down-regulation of cell surface receptors, but occurs at a postbinding step in hormone action. Here we present evidence that IGF-I and IGF-II also induce desensitization to the actions of both IGFs and insulin and that this effect is mediated by IGF receptors. First, the concentration dependence for this effect is similar to that for the IGF-I and IGF-II induction of tyrosine aminotransferase, which has been shown to be mediated by IGF receptors. Second, antibody to the insulin receptor, which blocks insulin, but not IGF-II, binding to HTC cells, causes a rightward shift in the concentration dependence for insulin induction of desensitization, but does not significantly change the concentration dependence for IGF-II. These results indicate that IGF-II-induced desensitization of IGF-II is not mediated by the insulin receptor, but presumably by an IGF receptor. Although the IGFs do cause a moderate decrease in the binding of IGFs and insulin (to .apprx. 50-75% of the control value), this cannot account for the virtually complete desensitization to their actions. We conclude that IGFs, like insulin, induce a nearly complete loss of responsiveness to insulin and IGFs, that this effect is mediated via IGF receptors, and that desensitization occurs to a step distal to hormone binding which may be common to the actions of insulin and the IGFs.

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Berg, Na, 1981: Resources Conservation Act: new window on the future. Economics ethics ecology: roots of productive conservation based on material presented at the 35th annual meeting of the Soil Conservation Society of America August 4 August 6-1980 Dearborn Michigan edited by Walter E Jaske: 141

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Resnick, Michael A.; Inga, Alberto, 2003: Functional mutants of the sequence-specific transcription factor p53 and implications for master genes of diversity. There are many sources of genetic diversity, ranging from programmed mutagenesis in antibody genes to random mutagenesis during species evolution or development of cancer. We propose that mutations in DNA sequence-specific transcription factors th...

Martin, G. F.; Dom, R., 1971: Reticulospinal fibers of the Opossum, Didelphis virginiana.2. Course, caudal extent and distribution. J Comp Neurol, 141: 467-483

Zaleszkiewicz Leszek, 2004: Morphology and genesis of the sub-Quaternary surface in the area around Leczyce near Lebork. Przeglad Geologiczny 52(8-1): 700-701

Rushton, D. N., 2003: Functional electrical stimulation and rehabilitation: An hypothesis. Functional Electrical Stimulation (FES), used to mimic a weak or paralysed movement, sometimes is followed by a specific recovery of voluntary power in that movement. The mechanism by which this occurs is unclear, and the presumption has often bee...

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Flack J.R.; Manna S.; Moltz J.; Chacos N.; Capdevila J., 1983: Epoxy eicosatrienoic acids stimulate glucagon and insulin release from isolated rat pancreatic islets. Metapyrone and eicosatetraynoic acid but not indomethacin are effective inhibitors of the secretory response of isolated rat pancreatic islets to arginine and glucose. Epoxyeicosatrienoic acids, products of the cytochrome P-450-NADPH dependent ara...

Schmidt, M., 1932: Benthonische Reifeformen bei Protrachyceras vilan-ovae v. Mojs. Butll catal. Hist. nat. (2): 223-232