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Regulation of insulin like growth factor messenger rna in rat growth plate by growth hormone


, : Regulation of insulin like growth factor messenger rna in rat growth plate by growth hormone. Endocrinology 122(4): 1515-1520

The mechanism of action for the stimulatory effect of GH on longitudinal bone growth is not yet clarified. Several recent receptors indicate that GH has a direct effect at the site of the epiphyseal growth plate, as opposed to the somatomedin hypothesis which holds that the effect of GH is mediated by circulating insulin-like growth factors (IGFs). Using an RNA probe in a solution hybridization assay we investigated the presence of IGF-I mRNA in rat rib growth plate. Hypophysectomy resulted in a decrease in the number of IGF-I mRNA copies compared to that in normal rats. Replacement treatment with GH restored the number of transcripts in a specific and dose-dependent manner. The results show that GH regulates the level of IGF-I mRNA in rat rib growth plate and give further support to the hypothesis that locally produced IGF-I might contribute to the stimulatory effect of GH on longitudinal bone growth.

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Related references

Isgaard, J.; Möller, C.; Isaksson, O.G.; Nilsson, A.; Mathews, L.S.; Norstedt, G., 1988: Regulation of insulin-like growth factor messenger ribonucleic acid in rat growth plate by growth hormone. The mechanism of action for the stimulatory effect of GH on longitudinal bone growth is not yet clarified. Several recent reports indicate that GH has a direct effect at the site of the epiphyseal growth plate, as opposed to the somatomedin hypoth...

Reinecke, M.; Schmid, A.C.; Heyberger-Meyer, B.; Hunziker, E.B.; Zapf, J., 2000: Effect of growth hormone and insulin-like growth factor I (IGF-I) on the expression of IGF-I messenger ribonucleic acid and peptide in rat tibial growth plate and articular chondrocytes in vivo. Conflicting data exist as to whether insulin-like growth factor I (IGF-I) messenger RNA (mRNA) and peptide are expressed within chondrocytes. This question is pertinent to the mode of GH action on longitudinal bone growth. We have, therefore, inve...

Uchiyama, T.; Kaji, H.; Abe, H.; Chihara, K., 1994: Negative regulation of hypothalamic growth hormone-releasing factor messenger ribonucleic acid by growth hormone and insulin-like growth factor I. Increased growth hormone-releasing factor messenger ribonucleic acid (GRF mRNA) and decreased somatostatin (SRIF) mRNA levels have been reported in the hypothalamus of hypophysectomized rats as well as of dwarf mice. In order to elucidate the effe...

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Hanna, J.D.; Lei, C.M.; Han, V.K.M., 1996: Tibial epiphyseal growth plate insulin-like growth factor-I and insulin-like growth factor binding protein-3 gene expression in uremic growth hormone treated rats. Pediatric Research 39(4 PART 2): 362A

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