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Regulation of int gene transcription by bacterio phage gamma location of the rna start generated by an int constitutive mutation


, : Regulation of int gene transcription by bacterio phage gamma location of the rna start generated by an int constitutive mutation. Journal of Molecular Biology 146(1): 157-166

The pI promoter of bacteriophage .lambda. is positively regulated by the phage eII and eIII proteins to provide for expression of the .lambda.int gene and differential regulation of int with respect to the adjacent xis gene. A sequence overlapping the start of the xis gene was identified with pI, based on the DNA sequence of this region and the sequence changes associated with regulatory mutations. One class of mutations, the intc point mutations, produce a high rate of int gene expression in the absence of eII/EIII in vivo and generate a transcription start site in vitro not found in wild type .lambda. 3 of these mutations alter the same base in the Pribnow box (-10 region) of the proposed pI sequence. The 5' terminal sequence of the RNA transcribed in vitro from an intc DNA template was analyzed and this RNA initiates with UTP at either of 2 adjacent bases, 9 and 10 base pairs downstream from the center of the proposed Pribnow box of pI. The proposed pI sequence can serve as a constitutive promoter in vitro when altered by the intc mutations. These results support the identification of the wild type sequence as the cII-activated pI promoter.

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