+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of interferon receptor expression in human blood lymphocytes in vitro and during interferon therapy

, : Regulation of interferon receptor expression in human blood lymphocytes in vitro and during interferon therapy. Journal of Clinical Investigation 77(5): 1632-1638

Interferons (IFN) elicit antiviral and antineoplastic activities by binding to specific receptors on the cell surface. The binding characteristics of IFN to human lymphocytes were studied using IFN .alpha.2 labeled with 125I to high specific activity. The specific binding curves generated were analyzed by the LIGAND program of Munson and Rodbard to determine receptor numbers. The number of receptors in peripheral blood lymphocytes (PBL) and tonsillar B-lymphocytes (TBL) from normal individuals were 505 .+-. 293 (n=10) and 393 .+-. 147 (n=3) resepctively. When these cells were preincubated in vitro with unlabeled IFN .alpha.2, the receptor number decreased to 82 .+-. 45 and 61 .+-. 16 respectively. Receptor binding activities recovered gradually over a period of 72 h when the cells were incubated in IFN-free medium. This recovery of receptors could be blocked by the addition of actinomycin D to the incubation medium. A similar decrease in receptor expression was observed in vivo in PBL from patients being treated daily with 5 .times. 106 units/m2 per d of IFN .alpha.2 by subcutaneous injection, for acute lymphoblastic leukemia or papilloma virus infections. Receptor numbers of PBL in vivo were further reduced concurrent with the progression of IFN therapy. Thus the reduction in IFN receptor expression observed in vitro can be demonstrated in vivo. These studies indicate that monitoring IFN receptor expression in vivo can provide information regarding the availability of IFN receptors at the cell surface for the mediation of IFN actions during the course of IFN therapy.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288797

PMID: 3009549

DOI: 10.1172/JCI112480

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Mainou Fowler, Tryfonia; Dignum, Helen; Taylor, Penelope R. A.; Dickinson, Anne M.; Saunders, Peter W. G.; Proctor, Stephen J.; Summerfield, Geoffrey P., 2001: Quantification improves the prognostic value of CD38 expression in B cell chronic lymphocytic leukaemia. Recent studies have shown that CD38 expressed as a percentage of antigen positivity can predict prognosis and disease progression in BCLL. The present study examines whether quantification of CD38 density expressed as antibody binding capacity (AB...

Whitford, Walter, G.; Anderson, John; Rice, Patricia, M., 1997: Stemflow contribution to the 'fertile island' effect in creosotebush, Larrea tridentata. Stemflow, throughfall and bulk precipitation were collected on six creosotebushes (Larrea tridentata) during 18 events in the summer rainy season in the northern Chihuahuan Desert. The average stemflow was 16.8 +- 1.9%; throughfall averaged 64.7 +...

Swisher, D. W.; Sudduth, K. A.; Borgelt, S. C., 1999: Optical measurement of granular fertilizer flow rates for precision agriculture. An optical sensor to measure granular fertilizer flow in an air stream was designed, built, and laboratory tested. The sensor components included a laser line generator that transmitted light across a trapezoidal chamber to two 16-element photodio...

Mano, Tsutomu., 1991: Problems of brown bear management in Hokkaido. Biological Sciences (Tokyo). November; 434: 183-189

Oster V.R., 1973: Treatment of isolated osseous abscesses. Sovetskaya Meditsina 36(9): 94-97

Fisher, W., 1980: Chain saw safety. Bulletin Washington State University Cooperative Extension Service: 980 (0727)

Ton, Y.; Nilov, N.; Kopyt, M., 2001: Phytomonitoring: the new information technology for improving crop production. Phytomonitoring is the first practicable computerized technology for observing real-time situation as well as long-term development of plants in greenhouses. Being introduced between a grower and cultivated plants, the phytomonitoring system provi...

Mustranta, A.; Kiesvaara, M.; Kuusi, T., 1976: Pectolytic changes in the composition of cucumbers during the harvesting period. Determinations were made for total solids, pectin content and degree of esterification, pectin methylesterase activity, polygalacturonase inhibition, and consistency in cucumber fruits of several cvs, notably Superb OE, grown in Finland for 2 seas...

Flynn, Michael; Werth, Diane; Gudipati, Rao; Pedersen, Wes; Serota, Harvey; Johnson, W. B.; Gibson, Paul; Hamilton, William; Morton, David, 1993: Influence of heparin duration on coronary angiographic characteristics in patients receiving accelerated weight-adjustment rt-PA dosing for acute myocardial infarction. Circulation 88(4 PART 2): I201

Stadtmuller, W., 1978: Official rules of earnings in 1977 for agricultural equipment mechanics. Agrartechnik international8, 57(9): 46-47