EurekaMag.com logo
+ Translate

Regulation of interleukin 2 receptor expression interleukin 2 as an inducing signal for the expression of its own receptor on a murine t helper cell line


, : Regulation of interleukin 2 receptor expression interleukin 2 as an inducing signal for the expression of its own receptor on a murine t helper cell line. European Journal of Immunology 15(7): 723-727

The interleukin 2 receptor (IL 2R) expression of a terpolymer of GAT-specific T helper (Th) cell line, L14, was studied using 2 different rat monoclonal antibodies specific for the murine IL 2R, 7D4 and 5A2-2. L14 T cells expressed IL 2R transiently, but contrary to the general assumption that IL 2R expression is maintained only by periodic antigen stimulation, it was observed that IL 2 itself was able to regulate the level of IL 2R on L14 Th lymphocytes. In particular, it was found that L14 T cells, which had not been recently stimulated and which expressed a very low level of high affinity IL 2R, could be induced to exhibit a high level of this receptor after exposure to recombinant IL 2, in the absence of any specific signal. The Th cell line 52.3 used as control could be induced to express high levels of IL 2R only after exposure to GAT; nevertheless, IL 2 seems to play a critical role in the in vitro survival of the resting state of 52.3 T cells.

(PDF 0-2 workdays service)

Accession: 006288806

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Bismuth, G.; Moreau, J.L.; Sommé, G.; Duphot, M.; Dautry-Varsat, A.; Robb, R.J.; Théze, J., 1985: Regulation of interleukin 2 (IL2) receptor expression: IL2 as an inducing signal for the expression of its own receptor on a murine T helper cell line. The interleukin 2 receptor (IL2R) expression of a terpolymer of L-Glu60, L-Ala30, L-Tyr10 (GAT)-specific T helper (Th) cell line, L14, was studied using two different rat monoclonal antibodies specific for the murine IL2R, 7D4 and 5A2-2. L14 T cel...

Huntenburg C.C.; Lum L.; Walker D.; Dinarello C.A., 1990: Down regulation of interleukin 1 il 1 receptor expression on d10s a subcloned murine t helper cell line by il 1. Journal of Cellular Biochemistry Supplement (14 PART B): 34

Bismuth G.; Moreau J.L.; Somme G.; Duphot M.; Dautry Varsat A.; Robb R.J.; Theze J., 1985: Regulation of interleukin 2 receptor expression interleukin 2 induces the expression of its own receptor on t helper cell lines. Federation Proceedings 44(4): 941

Matsushima K.; Yodoi J.; Tagaya Y.; Oppenheim J.J., 1986: Down regulation of interleukin 1 receptor expression by interleukin 1 and fate of internalized iodine 125 labeled interleukin 1 beta in a human large of granular lymphocyte cell line. The regulation of interleukin 1 (IL 1) receptor expression on a human large granular lymphocyte cell line, YT, and fate of internalized 125I-labeled IL 1.beta. (125I-IL 1.beta.) were studied. YT cells were selected for this study, because this cel...

Kaye, J.; Gillis, S.; Mizel, S.B.; Shevach, E.M.; Malek, T.R.; Dinarello, C.A.; Lachman, L.B.; Janeway, C.A., 1984: Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. By using as an experimental system the induction of growth of a [mouse] cloned, antigen:Ia-reactive helper T cell line by an antigen receptor-specific monoclonal antibody, growth was demonstrated to require 2 essential cofactors, exogenously produ...

Yodoi, J.; Teshigawara, K.; Nikaido, T.; Fukui, K.; Noma, T.; Honjo, T.; Takigawa, M.; Sasaki, M.; Minato, N.; Et-Al, 1985: T cell growth factor interleukin 2 receptor inducing factors 1. regulation of interleukin 2 receptor on a natural killer like cell line yt cells. A continuous cell line (YT cells) with inducible receptor for T cell growth factor (TCGF)/interleukin 2 (IL 2) was established from a 15 yr old boy with acute lymphoblastic lymphoma and thymoma. YT cells were tetraploid, having 4q+ chromosomal mar...

Chitko-McKown, C.G.; Ruef, B.J.; Rice-Ficht, A.C.; Brown, W.C., 1995: Interleukin-10 downregulates proliferation and expression of interleukin-2 receptor p55 chain and interferon-gamma, but not interleukin-2 or interleukin-4, by parasite-specific helper T cell clones obtained from cattle chronically infected with Babesia bovis or Fasciola hepatica. Human recombinant interleukin-10 (IL-10) was previously shown to inhibit accessory cell (AC)-dependent proliferation of bovine parasite-specific T helper 1 (Th1), Th2, and Th0 cells in an IL-2-reversible manner (Brown, W.C., Woods, V.M., Chitko-Mc...

Tsukagoshi, H.; Shimizu, Y.; Horie, T.; Fukabori, Y.; Shimizu, Y.; Iwamae, S.; Hisada, T.; Ishizuka, T.; Iizuka, K.; Dobashi, K.; Mori, M., 1999: Regulation by interleukin-1beta of gene expression of bradykinin B1 receptor in MH-S murine alveolar macrophage cell line. The effects of recombinant murine interleukin (IL)-1beta on gene expression of murine bradykinin B1 receptor (BDKRB1) in MH-S murine alveolar macrophage cell line were evaluated. BDKRB1 mRNA expression in MH-S cells was increased by IL-1beta (1, 3...

Yodoi J.; Nikaido T.; Sabe H.; Teshigawara K.; Okada M.; Tagaya Y.; Ishida N.; Shimizu A.; Maeda M.; Uchiyama T.; Honjo T., 1985: Role of interleukin 2 receptor inducing factor in the constitutive interleukin 2 receptor gene expression in adult t cell leukemia. Federation Proceedings 44(3): 799

Mehraban, F.; Kasturi, S., 1995: Expression of a soluble interleukin-1 receptor cDNA in rabbit synovial cell-line in vitro results in blockade of interleukin-1 stimulated stromelysin mRNA up-regulation. Arthritis & Rheumatism 38(9 SUPPL ): S400