+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of intestinal goblet cell secretion 1. role of parasympathetic stimulation

, : Regulation of intestinal goblet cell secretion 1. role of parasympathetic stimulation. American Journal of Physiology 242(4): G370-G379

The in vivo effects of the parasympathomimetic drug pilocarpine on rat intestinal goblet cells were analyzed by autoradiography, light microscopy (LM) and EM. Pilocarpine accelerated the release of mucus by compound exocytosis from crypt (but not surface) goblet cells throughout the small and large intestine. Pilocarpine-induced mucus secretion was blocked by atropine alone in ileum and colon, total inhibition in proximal small intestine required a combination of atropine and tubocurarine. The sensitivity of morphological-autoradiographic methods for detection of goblet cell secretion was compared with that of a biochemical detection method, separation of labeled high-MW glycoproteins by Sepharose 4B gel filtration of luminal washings. Even when secretion of labeled mucus by compound exocytosis was demonstrated by LM, EM and autoradiography gel filtration assay of luminal washings from pilocarpine-injected rats did not reveal an increase in labeled high-MW glucoproteins. Autoradiographs of mucosal tissue after luminal washing showed that newly secreted, labeled mucus was retained in the crypts and was unavailable to the biochemical assay. Direct observation of exocytosis in individual goblet cells apparently provided a qualitative but sensitive assay for short-term acceleration of intestinal mucus secretion.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006288829

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Maximow, A.A.; Bloom, W., 1948: A textbook of histology. Although a considerable amount of new material has been incorporated, the text occupies 661 pages as compared with 663 pages of the previous edition. There are now two columns of print to a page, and the book seems easier to read. The number of il...

Saha, A.K.; Smith, K.J.E.; Sue-Ling, H.; Sagar, P.M.; Burke, D.; Finan, P.J., 2012: Prognostic factors for survival after curative resection of Dukes' B colonic cancer. Data on the prognostic factors for survival in patients with locally advanced, node-negative colon cancer are limited. This study aimed to determine which factors might predict survival in patients with Dukes' B (T3 or T4, N0) colon cancer. O...

Nadkarni, P.M.; Banks, A.; Montgomery, K.; LeBlanc-Stracewski, J.; Miller, P.; Krauter, K., 1996: CONTIG EXPLORER: interactive marker-content map assembly. In STS-content mapping of a region, multiple optimal or near-optimal putative orders of markers exist. Determining which of the markers in this region can be placed reliably on the physical map of the chromosome and which markers lack sufficient e...

Daniel Martínez, P., 1974: The habitat of the underfed. Gaceta Medica de Mexico 107(4): 268-273

Tse, G.M.; Tan, P-Hoon.; Lacambra, M.D.; Jara-Lazaro, A.Richelia.; Chan, S-Ki.; Lui, P.C.; Ma, T.K.F.; Vong, J.S.; Ng, D.C.H.; Shi, H-Juan.; Lam, W.W., 2011: Papillary lesions of the breast--accuracy of core biopsy. To assess the accuracy of diagnosing papillary breast lesions in core needle biopsy. One hundred biopsy specimens of papillary breast lesions were reviewed and compared with the final excisional diagnoses. The discordant biopsy specimens were stai...

BESSIERE.E.; VERIN.P.; LEGOFF.J.; LEREBELLER.M.J., 1964: On Some Familial Associations Of Skeletel Abnormalities And Abnormalities Of The Anterior Segment Of The Eye. Bulletin des Societes D'ophtalmologie de France 64: 637-642

Rasgon, B.M.; Cruz, R.M.; Hilsinger, R.L.; Korol, H.W.; Callan, E.; Wolgat, R.A.; Selby, J.V., 1991: Infiltration of epinephrine in tonsillectomy: a randomized, prospective, double-blind study. A variety of hemostatic agents and techniques have been used in an attempt to reduce intraoperative blood loss and postoperative bleeding in tonsillectomy. The efficacy of most of these substances and techniques has been judged primarily on clinic...

Flores, J.; Shiiya, N.; Takashi, K.; Yoshimoto, K.; Matsuzaki, K.; Yamashita, T.; Tanino, M.; Yasuda, K., 2005: True aneurysm of an ilio-femoral saphenous vein graft. Journal of Cardiovascular Surgery 46(2): 182-183

Sina, S.; Zeinali, B.; Karimipoorfard, M.; Lotfalizadeh, F.; Sadeghi, M.; Zamani, E.; Faghihi, R., 2015: Investigation of the entrance surface dose and dose to different organs in lumbar spine imaging. Dose assessment using proper dosimeters is especially important in radiation protection optimization and imaging justification in diagnostic radiology. The aim of this study is to obtain the Entrance Skin Dose (ESD) of patients undergoing lumbar s...

Heslenfeld, P..; Lievin, J..; Mielchen, V..; Pickaver, A..; Salman, A.., 2003: Corridors and ecosystems: coastal and marine areas. Council of Europe Nature and Environment Series, 134: 1-46