EurekaMag.com logo
+ Translate

Regulation of intestinal goblet cell secretion 1. role of parasympathetic stimulation


, : Regulation of intestinal goblet cell secretion 1. role of parasympathetic stimulation. American Journal of Physiology 242(4): G370-G379

The in vivo effects of the parasympathomimetic drug pilocarpine on rat intestinal goblet cells were analyzed by autoradiography, light microscopy (LM) and EM. Pilocarpine accelerated the release of mucus by compound exocytosis from crypt (but not surface) goblet cells throughout the small and large intestine. Pilocarpine-induced mucus secretion was blocked by atropine alone in ileum and colon, total inhibition in proximal small intestine required a combination of atropine and tubocurarine. The sensitivity of morphological-autoradiographic methods for detection of goblet cell secretion was compared with that of a biochemical detection method, separation of labeled high-MW glycoproteins by Sepharose 4B gel filtration of luminal washings. Even when secretion of labeled mucus by compound exocytosis was demonstrated by LM, EM and autoradiography gel filtration assay of luminal washings from pilocarpine-injected rats did not reveal an increase in labeled high-MW glucoproteins. Autoradiographs of mucosal tissue after luminal washing showed that newly secreted, labeled mucus was retained in the crypts and was unavailable to the biochemical assay. Direct observation of exocytosis in individual goblet cells apparently provided a qualitative but sensitive assay for short-term acceleration of intestinal mucus secretion.

(PDF 0-2 workdays service)

Accession: 006288829

PMID: 7065259

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Specian, R.D.; Neutra, M.R., 1982: Regulation of intestinal goblet cell secretion. I. Role of parasympathetic stimulation. The in vivo effects of the parasympathomimetic drug pilocarpine on rat intestinal goblet cells were analyzed by autoradiography, light microscopy (LM), and electron microscopy (EM). Pilocarpine accelerated the release of mucus by compound exocytos...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. Electrical field stimulation in vitro. American Journal of Physiology 247: 82-7

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. IV. Electrical field stimulation in vitro. To determine whether transmitters released from enteric neurons can elicit secretion from goblet cells, full-thickness sheets of adult rat distal ileum or descending colon were mounted in modified Ussing chambers, and mucus secretion was assessed...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion 4. electrical field stimulation in vitro. Whether transmitters released from enteric neurons can elicit secretion from goblet cels was studied using full-thickness sheets of adult rat distal ileum of descending colon mounted in modified Ussing chambers. Mucus secretion was assessed morpho...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. Isolated intestinal epithelium. American Journal of Physiology 247: 74-81

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. III. Isolated intestinal epithelium. Cholinergic secretagogues evoke mucus secretion from goblet cells in the crypts of small and large intestinal mucosa in vivo and in organ culture. It was not known whether this response reflected a direct action on epithelial cell receptors or an...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion 3. isolated intestinal epithelium. Cholinergic secretagogues evoke mucus secretion from goblet cells in the crypts of small and large intestinal mucosa in vivo and in organ culture. It was not known whether this response reflected a direct action on epithelial cell receptors or an...

Neutra, M.R.; O'malley, L.J.; Specian, R.D., 1982: Regulation of intestinal goblet cell secretion 2. a survey of potential secretagogues. The factors that regulate the rate of mucus secretion in [rabbit] intestinal goblet cells are only partially defined. Autoradiographic and ultrastructural studies demonstrated that muscarinic cholinergic agents accelerate the exocytosis of mucus f...

Neutra, M.R.; O'Malley, L.J.; Specian, R.D., 1982: Regulation of intestinal goblet cell secretion. II. A survey of potential secretagogues. The factors that regulate the rate of mucus secretion in intestinal goblet cells are only partially defined. Autoradiographic and ultrastructural studies demonstrated that muscarinic cholinergic agents accelerate the exocytosis of mucus from goble...

Hayashi, D.; Li, D.; Hayashi, C.; Shatos, M.; Hodges, R.R.; Dartt, D.A., 2012: Role of histamine and its receptor subtypes in stimulation of conjunctival goblet cell secretion. The purpose of this study was to determine the effect of histamine and its receptors on goblet cell secretion. Cultured rat and human goblet cells were grown in RPMI 1640. Goblet cell secretion of high molecular weight glycoconjugate was measured...