EurekaMag.com logo
+ Translate

Regulation of intestinal goblet cell secretion 2. a survey of potential secretagogues


, : Regulation of intestinal goblet cell secretion 2. a survey of potential secretagogues. American Journal of Physiology 242(4): G380-G387

The factors that regulate the rate of mucus secretion in [rabbit] intestinal goblet cells are only partially defined. Autoradiographic and ultrastructural studies demonstrated that muscarinic cholinergic agents accelerate the exocytosis of mucus from goblet cells in the crypts throughout the small and large intestine, both in vivo and in mucosal organ culture. Other factors that may alter mucus secretory rates were studied. Mucosal explants were exposed to potential secretagogues and inhibitors in the organ-culture system and analyzed by light microscopy and EM. .alpha.- and .beta.-Adrenergic agents, gastrointestinal regulatory peptides, serotonin, histamine, and dibutyryl cyclic nucleotides were tested over wide concentration ranges. With the exception of histamine, none of these agents accelerated or inhibited the exocytosis of mucous granules. Histamine was effective at the concentration of 10-4 M and induced rapid, compound exocytosis by crypt goblet cells in mucosal explants from the colon but not from small intestine. The response to histamine was unaffected by atropine. Goblet cells on the mucosal surface released mucus by compound exocytosis when exposed to mustard oil, a nonspecific chemical irritant, but not when exposed to histamine or cholinergic agents.

(PDF 0-2 workdays service)

Accession: 006288830

PMID: 7065260

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Neutra, M.R.; O'Malley, L.J.; Specian, R.D., 1982: Regulation of intestinal goblet cell secretion. II. A survey of potential secretagogues. The factors that regulate the rate of mucus secretion in intestinal goblet cells are only partially defined. Autoradiographic and ultrastructural studies demonstrated that muscarinic cholinergic agents accelerate the exocytosis of mucus from goble...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion 3. isolated intestinal epithelium. Cholinergic secretagogues evoke mucus secretion from goblet cells in the crypts of small and large intestinal mucosa in vivo and in organ culture. It was not known whether this response reflected a direct action on epithelial cell receptors or an...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. Isolated intestinal epithelium. American Journal of Physiology 247: 74-81

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. III. Isolated intestinal epithelium. Cholinergic secretagogues evoke mucus secretion from goblet cells in the crypts of small and large intestinal mucosa in vivo and in organ culture. It was not known whether this response reflected a direct action on epithelial cell receptors or an...

Specian, R.D.; Neutra, M.R., 1982: Regulation of intestinal goblet cell secretion 1. role of parasympathetic stimulation. The in vivo effects of the parasympathomimetic drug pilocarpine on rat intestinal goblet cells were analyzed by autoradiography, light microscopy (LM) and EM. Pilocarpine accelerated the release of mucus by compound exocytosis from crypt (but not...

Specian, R.D.; Neutra, M.R., 1982: Regulation of intestinal goblet cell secretion. I. Role of parasympathetic stimulation. The in vivo effects of the parasympathomimetic drug pilocarpine on rat intestinal goblet cells were analyzed by autoradiography, light microscopy (LM), and electron microscopy (EM). Pilocarpine accelerated the release of mucus by compound exocytos...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. IV. Electrical field stimulation in vitro. To determine whether transmitters released from enteric neurons can elicit secretion from goblet cells, full-thickness sheets of adult rat distal ileum or descending colon were mounted in modified Ussing chambers, and mucus secretion was assessed...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. Electrical field stimulation in vitro. American Journal of Physiology 247: 82-7

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion 4. electrical field stimulation in vitro. Whether transmitters released from enteric neurons can elicit secretion from goblet cels was studied using full-thickness sheets of adult rat distal ileum of descending colon mounted in modified Ussing chambers. Mucus secretion was assessed morpho...

Ogata, H.; Sands, B.E.; Podolsky, D.K., 1995: Regulation of expression of intestinal trefoil factor Analysis of intestinal goblet cell specific transcriptional elements. Gastroenterology 108(4 SUPPL ): A312