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Regulation of intestinal goblet cell secretion 3. isolated intestinal epithelium


, : Regulation of intestinal goblet cell secretion 3. isolated intestinal epithelium. American Journal of Physiology 247(6 PART 1): G674-G681

Cholinergic secretagogues evoke mucus secretion from goblet cells in the crypts of small and large intestinal mucosa in vivo and in organ culture. It was not known whether this response reflected a direct action on epithelial cell receptors or an indirect effect involving intermediate neurons of the enteric nervous system. To resolve this, carbachol was applied to isolated rat intestinal epithelium maintained in vitro. Intact sheets of epithelium, measuring 10-200 mm2, were isolated from the ileum and colon of adult rats following short intravascular perfusion with 30 mM EDTA. The isolated epithelia lacked a basal lamina and cytoplasmic blebs formed on the basal cell surfaces, but cell ultrastructure was normal and intercellular injections were intact. Autoradiography revealed that both goblet and columnar cells continued to incorporate [3H]glucosamine into nascent secretory macromolecules for at least 45 min after isolation. When exposed to 20 .mu.M carbachol for 5 min, crypt goblet cells discharged their stored mucin granules by compound exocytosis, whereas goblet cells in portions of the epithelium derived from villi or mucosal surfaces were unresponsive. Cholinergic secretagogues evidently act directly on crypt epithelial cells to elicit mucus secretion.

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Accession: 006288831

PMID: 6391203

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Related references

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. Isolated intestinal epithelium. American Journal of Physiology 247: 74-81

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. III. Isolated intestinal epithelium. Cholinergic secretagogues evoke mucus secretion from goblet cells in the crypts of small and large intestinal mucosa in vivo and in organ culture. It was not known whether this response reflected a direct action on epithelial cell receptors or an...

Neutra, M.R.; Phillips, T.L.; Phillips, T.E., 1984: Regulation of intestinal goblet cells in situ, in mucosal explants and in the isolated epithelium. Cholinergic secretagogues were previously shown to accelerate mucin secretion from intestinal goblet cells of adult rats and rabbits, both in vitro and in mucosal explants. This rapid secretory response occurs only in crypt cells; surface goblet c...

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Neutra, M.R.; O'Malley, L.J.; Specian, R.D., 1982: Regulation of intestinal goblet cell secretion. II. A survey of potential secretagogues. The factors that regulate the rate of mucus secretion in intestinal goblet cells are only partially defined. Autoradiographic and ultrastructural studies demonstrated that muscarinic cholinergic agents accelerate the exocytosis of mucus from goble...

Specian, R.D.; Neutra, M.R., 1982: Regulation of intestinal goblet cell secretion. I. Role of parasympathetic stimulation. The in vivo effects of the parasympathomimetic drug pilocarpine on rat intestinal goblet cells were analyzed by autoradiography, light microscopy (LM), and electron microscopy (EM). Pilocarpine accelerated the release of mucus by compound exocytos...

Specian, R.D.; Neutra, M.R., 1982: Regulation of intestinal goblet cell secretion 1. role of parasympathetic stimulation. The in vivo effects of the parasympathomimetic drug pilocarpine on rat intestinal goblet cells were analyzed by autoradiography, light microscopy (LM) and EM. Pilocarpine accelerated the release of mucus by compound exocytosis from crypt (but not...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. IV. Electrical field stimulation in vitro. To determine whether transmitters released from enteric neurons can elicit secretion from goblet cells, full-thickness sheets of adult rat distal ileum or descending colon were mounted in modified Ussing chambers, and mucus secretion was assessed...

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion. Electrical field stimulation in vitro. American Journal of Physiology 247: 82-7

Phillips, T.E.; Phillips, T.H.; Neutra, M.R., 1984: Regulation of intestinal goblet cell secretion 4. electrical field stimulation in vitro. Whether transmitters released from enteric neurons can elicit secretion from goblet cels was studied using full-thickness sheets of adult rat distal ileum of descending colon mounted in modified Ussing chambers. Mucus secretion was assessed morpho...