+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of intestinal goblet cell secretion 3. isolated intestinal epithelium

American Journal of Physiology 247(6 PART 1): G674-G681

Regulation of intestinal goblet cell secretion 3. isolated intestinal epithelium

Cholinergic secretagogues evoke mucus secretion from goblet cells in the crypts of small and large intestinal mucosa in vivo and in organ culture. It was not known whether this response reflected a direct action on epithelial cell receptors or an indirect effect involving intermediate neurons of the enteric nervous system. To resolve this, carbachol was applied to isolated rat intestinal epithelium maintained in vitro. Intact sheets of epithelium, measuring 10-200 mm2, were isolated from the ileum and colon of adult rats following short intravascular perfusion with 30 mM EDTA. The isolated epithelia lacked a basal lamina and cytoplasmic blebs formed on the basal cell surfaces, but cell ultrastructure was normal and intercellular injections were intact. Autoradiography revealed that both goblet and columnar cells continued to incorporate [3H]glucosamine into nascent secretory macromolecules for at least 45 min after isolation. When exposed to 20 .mu.M carbachol for 5 min, crypt goblet cells discharged their stored mucin granules by compound exocytosis, whereas goblet cells in portions of the epithelium derived from villi or mucosal surfaces were unresponsive. Cholinergic secretagogues evidently act directly on crypt epithelial cells to elicit mucus secretion.

(PDF 0-2 workdays service: $29.90)

Accession: 006288831

PMID: 6391203

Related references

Regulation of intestinal goblet cells in situ, in mucosal explants and in the isolated epithelium. Ciba Foundation Symposium 109: 20-39, 1984

Regulation of intestinal goblet cell secretion 2. a survey of potential secretagogues. American Journal of Physiology 242(4): G380-G387, 1982

Regulation of intestinal goblet cell secretion 1. role of parasympathetic stimulation. American Journal of Physiology 242(4): G370-G379, 1982

Regulation of intestinal goblet cell secretion 4. electrical field stimulation in vitro. American Journal of Physiology 247(6 PART 1): G682-G687, 1984

Regulation of expression of intestinal trefoil factor Analysis of intestinal goblet cell specific transcriptional elements. Gastroenterology 108(4 SUPPL ): A312, 1995

Ultrastructural features of the intestinal epithelium I The differentiated goblet cell. 1962

The effect of ATP, ADP and AMP on intestinal goblet cell mucin secretion. Journal of Pharmacy & Pharmacology 46(SUPPL 2): 1068, 1994

Paneth and goblet cell-mediated protection of the intestinal epithelium during methotrexate-induced mucositis. Gastroenterology 118(4 Suppl 2 Part 1): AGA A826, April, 2000

Secretion of intestinal ferments in intestines isolated according to Tiri and in denervated intestinal fragments from dogs and its relation to the quantity of protein in food. II. Secretion of intestinal ferments and their excretion in feces. Voprosy Pitaniia 14(5): 20-27, 1955

Secretion of intestinal enzymes in intestinal segments isolated by the Thiry method, and in denervated segments, in the dog, in relation to amount of dietary protein. 1. Quantity of intestinal secretion. Vop. Pitan, 14: 3, 16-22, 1955