+ Translate

Regulation of intra cellular pyro phosphatase ec activity and conservation of the phospho anhydride energy of inorganic pyro phosphate in microbial metabolism

, : Regulation of intra cellular pyro phosphatase ec activity and conservation of the phospho anhydride energy of inorganic pyro phosphate in microbial metabolism. Zeitschrift fuer Naturforschung Section C Journal of Biosciences 31(9-10): 544-550

The conservation of the phosphoanhydride-energy of PPi in microbial metabolism requires a stringent metabolic control of the intracellular pyrophosphatases (PPases, EC The rate of intracellular PPi-liberation during biosynthesis of cellular constituents is calculated from the specific growth rate and the macromolecular composition of the respective microorganism. This rate is compared with the maximal specific activity of PPase in cell free extracts or purified enzyme preparations to study the possibility of the limitation of biosynthesis through PPase-activity. The catalytic and regulatory properties of microbial PPases are discussed in respect to the occurrence of PPi-dependent transphosphorylases. Certain anaerobic microorganisms including photosynthetic bacteria can use PPi instead of ATP as phosphate donor for transphosphorylation reactions.

(PDF 0-2 workdays service)

Accession: 006288847

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Horder M., 1973: Inorganic pyro phosphate phospho hydrolytic activity in human serum catalytic properties of the ionic species of inorganic pyro phosphate and magnesium pyro phosphate complexes. Biochimica et Biophysica Acta 321(1): 329-335

Anonymous, 1972: Biochemical and clinical aspects of pyro phosphate metabolism part 5 the activity of inorganic pyro phosphatase ec 3611 in the kidneys of man and rat in the presence of different ortho phosphate intakes

Nordlie R.C., 1969: Effects of cetyltrimethyl ammonium bromide on catalytic properties of kidney microsomal glucose 6 phosphatase inorganic pyro phosphate glucose phospho transferase and inorganic pyro phosphatase activities. Biochimica Et Biophysica Acta: 636-643

Jensen K.F.; Houlberg U.; Nygaard P., 1979: Thin layer chromatographic methods to isolate phosphorus 32 labeled 5 phospho ribosyl alpha 1 pyro phosphate determination of cellular 5 phospho ribosyl alpha 1 pyro phosphate pools and assay of 5 phospho ribosyl alpha 1 pyro phosphate synthetase activity. Conditions are described where 5-phosphoribosyl-.alpha.-1-pyrophosphate (PRPP) can be determined by TLC methods commonly used for the determination of nucleoside triphosphate pools in 32P-labeled bacteria. A 2-dimensional chromatographic system is...

Anonymous, 1975: Enzyme catalyzed hydrolysis of inorganic pyro phosphate current view of problems in characterization of inorganic pyro phosphate phospho hydrolytic activity associated with alkaline phosphatases ec 3131

Anonymous, 1973: Inorganic pyro phosphatase ec 3611 and photosynthesis by isolated chloroplasts part 1 characterization of chloroplast pyro phosphatase and its relation to the response to exogenous pyro phosphate

Haromy T.P.; Knight W.B.; Dunaway Mariano D.; Sundaralingam M., 1982: X ray crystallographic and nmr spectral studies of the products from the yeast inorganic pyro phosphatase cobalt tetra ammine pyro phosphate reaction investigation of the pyro phosphatase reaction mechanism. Yeast inorganic pyrophosphatase catalyzes the hydrolysis of P1,P2-bidentate Co(NH3)4 pyrophosphate [Co-(NH3)4PP] to the cis, bis(phosphate)complex Co(NH3)4(Pi)2, which is not stable at neutral pH and over a period of 24 h converts to HPO42- and a...

Anonymous, 1970: Studies on plasma membranes part 11 inorganic pyro phosphatase ec 3611 inorganic pyro phosphate glucose phospho transferase and glucose 6 phosphatase ec 3139 in plasma membranes and microsomes isolated from rat and mouse livers and hepatomas

Khandelwal, R.L., 1978: The regulation of liver phospho protein phosphatase by inorganic pyro phosphate and cobalt. The preincubation of rat liver crude extracts with ATP caused a 60% inactivation of phosphoprotein phosphatase in 30 min at C. The presence of Mg2+, or cyclic AMP, along with ATP in the preincubation mixture had no effect on the inactiv...

Springs, B.; Cooperman, B.S.; Welsh, K.M., 1981: Thermodynamics kinetics and mechanism in yeast inorganic pyro phosphatase ec catalysis of inorganic pyro phosphate inorganic phosphate equilibration. Two methods for quantitatively measuring PPi in the presence of 103-104 M excesses of Pi were developed and used to measure the extent of enzyme-bound pyrophosphate (EPPi) formation in solutions of yeast inorganic pyrophosphatase and Pi. The rate...