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Regulation of l amino acid oxidase and d amino acid oxidase in neurospora crassa


, : Regulation of l amino acid oxidase and d amino acid oxidase in neurospora crassa. Molecular & General Genetics 186(1): 33-39

N. crassa possesses an inducible L-amino acid oxidase that is expressed only when cells are derepressed for N in the presence of an amino acid. Enzyme synthesis requires both induction by an amino acid and simultaneous N catabolite derepression. C limitation in the presence of an amino acid does not permit induction of L-amino acid oxidase. The nit-2 gene is a major regulatory locus which is believed to mediate N catabolite repression in Neurospora. Mutants of nit-2 are repressed for L-amino acid oxidase activity under conditions which lead to good enzyme induction in wild type and nit-2 revertants. The loss of the enzyme in nit-2 mutants does not result from inducer exclusion, which suggests that the nit-2 gene product has a direct role in controlling the expression of this enzyme. Substantial amounts of L-amino acid oxidase were detected in the growth medium as well as in cell extracts of the wild type strain. Biochemical data indicates that the intracellular and the extracellular L-amino acid oxidases are identical. Inhibitors of protein and of RNA synthesis block accumulation of L-amino acid oxidase, suggesting that enzyme expression is controlled at the level of transcription. D-amino acid oxidase can be detected in cell extracts of Neurospora grown in the presence of a D-amino acid. The enzyme is present in cys-3 mutants and is not repressed by high concentrations of sulfate or N indicating that D-amino acid oxidase is not a member of the S or N regulatory circuits of this organism.

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Accession: 006288914

PMID: 6125872

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Related references

Bender, A.E.; Krebs, H.A., 1950: The oxidation of various synthetic -amino-acids by mammalian D-amino-acid oxidase, L-amino-acid oxidase of cobra venom and the L- and D-amino-acid oxidase of Neurospora crassa. The rate of oxidation was examined of 48 alpha-amino acids (among them 27 synthetic compounds not previously tested) in the presence of crude prepns. of the D-amino-acid oxidase of sheep kidney, of the L-amino acid oxidase of cobra venom and the L...

Bender, A.E.; Krebs, H.A., 1950: The oxidation of various synthetic alpha-amino-acids by mammalian d-amino-acid oxidase, l-amino-acid oxidase of cobra venom and the l- and d-amino-acid oxidases of Neurospora crassa. Biochemical Journal 46(2): 210-219

Sikora, L.; Marzluf, G.A., 1982: Regulation of L-amino acid oxidase and of D-amino acid oxidase in Neurospora crassa. Neurospora crassa possesses an inducible L-amino acid oxidase that is expressed only when cells are derepressed for nitrogen in the presence of an amino acid. Enzyme synthesis requires both induction by an amino acid and simultaneous nitrogen cata...

Sikora, L.M.rzluf, G., 1982: Regulation of L-amino acid axidase and of D-amino acid oxidase in Neurospora crassa Fungi. MGG molecular and general genetics86(1): 33-39

Niedermann, D.M.; Lerch, K., 1991: Regulation of biosynthesis of L-amino acid oxidase by Neurospora crassa. L-Amino acid oxidase was purified from liquid cultures of Neurospora crassa induced by L-phenylalanine, D-phenylalanine, ATP and cycloheximide. Although the four enzyme species isolated were found to differ in size and electrophoretic mobility, th...

Calderón, J.; Olvera, L.; Martínez, L.M.; Dávila, G., 1997: A Neurospora crassa mutant altered in the regulation of L-amino acid oxidase. The isolation and characterization of a Neurospora crassa mutant altered in L-amino oxidase regulation is reported. The previously isolated gln-1bR8 strain, which only synthesizes the glutamine synthetase alpha monomer and lacks the beta monomer,...

Aurich, H.; Tauchert, H.; Schücker, G.; Luppa, D., 1972: Interactions between the amino acid pool and L-amino-acid oxidase in Neurospora crassa. Zeitschrift für Allgemeine Mikrobiologie 12(7): 529-535

Bender, A.E.; Krebs, H.A.; Horowitz, N.H., 1949: Amino acid oxidase of Neurospora crassa. Biochemical Journal 45(4): Suppl, Xxi-Suppl, Xxi

Aurich H.; Tauchert H., 1971: Repression of 1 amino acid oxidase from neurospora crassa by biotin. Acta Biologica et Medica Germanica 27(1): 203-205

Debusk R.M.; Ogilvie S., 1983: Extracellular l amino acid oxidase activity in neurospora crassa. Abstracts of the Annual Meeting of the American Society for Microbiology 83: K262