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Regulation of l asparaginase formation by amino acids in bacillus mesentericus






Mikrobiologiya 45(2): 259-265

Regulation of l asparaginase formation by amino acids in bacillus mesentericus

The role of amino acids in regulation of L-asparaginase formation was studied in B. mesentericus 43A. Aspartic acid and, to a less extent, asparagine repress biosynthesis of the enzyme. Glutamic acid, glutamine and 15 other amino acids, added separately at a concentration of 10 or 20 mM to the growing culture, have no effect on the enzyme activity. Addition of a combination of all 18 amino acids, each at a concentration of 4 mM, to the culture represses the activity by 64%; addition of an acid hydrolysate of lactalbumin (10 g/l) represses the enzyme activity by 80%. A mixture of amino acids without aspartic acid and asparagine also displays a strong repressing action. Amino acids formed from aspartic acid.sbd.lysine, methionine and isoleucine.sbd.do not repress enzyme biosynthesis together or separately. Ammonium N also does not regulate asparaginase formation. The cumulative repressing action of amino acids is probably manifested via catabolite repression.


Accession: 006288919



Related references

Tyul'panova E.S.; Eremenko V.V., 1976: Regulation of l asparaginase production in bacillus mesentericus by amino acids. Microbiology (New York) 45(2): 229-234

Tiul'panova, E.S.; Eremenko, V.V., 1976: Amino acid regulation of L-asparaginase formation in Bacillus mesentericus. The role of amino acids in regulation of L-asparaginase formation was studied in Bacillus mesentericus 43A. Asparagic acid and, to a less extent, asparagine repress biosynthesis of the enzyme. Glutamic acid, glutamine, and other 15 studied amino a...

Eremenko, V.V.; Mardashev, S.R.; Tyul'panova, E.S.; Evseev, L.P., 1976: Regulation of l asparaginase ec 3.5.1.1 biosynthesis in mutants of bacillus mesentericus 43a growing poorly on aspartic acid. Biosynthesis of L-asparaginase (EC 3.5.1.1.) was inhibited in the growing culture of B. mesentericus 43A by addition of L-aspartic acid (20 mM). Treatment with methyl nitrosourea (2 mg/ml) produced mutants which grew poorly on aspartic acid as sol...

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Kolev D.A., 1984: Repression of the synthesis of exoproteinases in bacillus mesentericus strain 90 r form by amino acids. Beshkov, M (Ed ) Ispekhi Na Promishchlenata I Infektsioziata Mikroblioliya; Achievements Of Industrial And Infectious Microbiology; 4th Jubilee Scientific Session, June 13-14, 1983 318p Bulgarian Academy Of Sciences: Sofia, Bulgaria Illus : 39-44

Tiul'panova, E.S.; Eremenko, V.V.; Mardashev, S.R., 1972: Activity and properties of L-asparaginase from Bacillus mesentericus 43A. Mikrobiologiia 41(3): 423-429

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