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Regulation of l phenyl alanine ammonia lyase ec 4.3.1.5 in germinating lettuce lactuca sativa seeds effects of substrate analogs and phenyl propanoid compounds


Zeitschrift fuer Pflanzenphysiologie 114(2): 163-172
Regulation of l phenyl alanine ammonia lyase ec 4.3.1.5 in germinating lettuce lactuca sativa seeds effects of substrate analogs and phenyl propanoid compounds
The effect of various substrate analogues and some of the intermediates and endproducts of the phenylpropanoid pathway on the development of L-phenylalanine ammonia-lyase (PAL) in relation to the growth of the embryonic axes in germinating lettuce seeds was investigated. Externally supplied L-phenylalanine and its D-isomer did not affect extractable PAL activity at 50 and 100 .mu.M concentrations, whereas at 500 .mu.M L-phenylalanine, there was a slight but significant inhibition of PAL activity. In the presence of 500 .mu.M D-phenylalanine a slight stimulation of PAL activity was observed. Two other substrate analogs, p-fluorophenylalanine and tryptophan, strongly inhibited both the growth of the radicle as well as the development of PAL activity, whereas tyrosine significantly stimulated both the radicle elongation and PAL activity. All the intermediates of the phenylpropanoid pathway used were inhibitory to growth and PAL activity, the order of effectiveness for inhibition being cinnamic acid > p-courmaric acid > ferulic acid > caffeic acid. Some of the end products of the phenylpropanoid pathway, i.e., coumarin, quercetin an chlorogenic acid were also very strong inhibitors both of radicle elongation and PAL activity. In all these treatments a strong correlation between PAL activity and radicle elongation was observed supporting the earlier observations that in germinating lettuce seeds any chemical or physical treatment that inhibits radicle elongation also inhibits PAL activity.


Accession: 006288929



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