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Regulation of lactic acid production by Leptotrichia buccalis


, : Regulation of lactic acid production by Leptotrichia buccalis. Caries Research 15(1): 21-25

Two lactate dehydrogenase (EC 1.1.1.27) species were separated by agarose A 0.5 M molecular exclusion column chromatography from cell-free extracts obtained from L. buccalis. Both lactate dehydrogenases were under negative control by ATP but the kinetic responses of the respective lactate dehydrogenase species to ATP were separate and distinct. The higher MW lactate dehydrogenase (LDH1) showed a negative hyperbolic kinetic response to ATP and Dixon plots of the ATP saturation data gave a Ki for ATP of 1.8 M. The activity of the lower MW lactate dehydrogenase (LDH2) was a negative sigmoidal function of the ATP concentration and had an [M]0.5V value for ATP of 2.5 M. A Hill coefficient of 1.95 was obtained from Hill plots of the ATP saturation data for LDH2, which indicated multiple ATP binding sites on the enzyme with some degree of cooperation interaction between them.

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Accession: 006288950

PMID: 6937247

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Related references

Gilmour, M.N.; Howell, A.; Bibby, B.G., 1961: The classification of organisms termed Leptotrichia (Leptothrix) buccalis. I. Review of the literature and proposed separation into Leptotrichia buccalis Trevisan, 1879 and Bacterionema gen. nov., B. matruchotti (Mendel, 1919) comb. nov. Bacteriological Reviews 25: 131-141

Girard A.E.; Jacius B.H., 1974: Fatty acid composition of leptotrichia buccalis and some fusobacterium sp. Abstracts of the Annual Meeting of the American Society for Microbiology 74: 46

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Eribe, E.R.K.; Paster, B.J.; Caugant, D.A.; Dewhirst, F.E.; Stromberg, V.K.; Lacy, G.H.; Olsen, I., 2004: Genetic diversity of Leptotrichia and description of Leptotrichia goodfellowii sp. nov., Leptotrichia hofstadii sp. nov., Leptotrichia shahii sp. nov. and Leptotrichia wadei sp. nov. Sixty strains of Gram-negative, anaerobic, rod-shaped bacteria from human sources initially assigned to Leptotrichia buccalis (n=58) and 'Leptotrichia pseudobuccalis' (n=2) have been subjected to polyphasic taxonomy. Full-length 16S rDNA...

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