EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of lambda dv plasmid DNA replication. A quantitative model for control of plasmid lambda dv replication in the bacterial cell division cycle


, : Regulation of lambda dv plasmid DNA replication. A quantitative model for control of plasmid lambda dv replication in the bacterial cell division cycle. Journal of Molecular Biology 191(3): 367-382

A quantitative model for the regulation of replication of plasmid .lambda.dv in the Escherichia coli cell division cycle has been developed. The regulatory processes include the interactions of cro repressor proteins with the rightward operator DNA sites, the transcriptional activation of the .lambda.dv replication origin, and the interaction of initiation proteins with activated origins to form functional replication initiation complexes. A statistical thermodynamic model was used to predict probable configurations of the regulatory processes in a single growing cell. These probabilities were coupled by a kinetic model to the events of the cell cycle such as initiation of mRNA transcription and protein translation and the initiation of plasmid DNA replication. Parameter values were chosen so that the simulated values for plasmid copy number and repressor and initiator protein concentrations of the model agreed with experimentally determined estimates. Simulated deviations from regular segregation of the various components at cell division, such as plasmid copies and free and bound repressor proteins, suggest that .lambda.dv replication control responds only slowly to these perturbations. The consequence of this slow response to perturbations, which are expected at a random frequency, was simulated for a population of .lambda.dv-containing cells in a growing culture. This simulation predicts instability of inheritance of .lambda.dv plasmids in the population, despite the very high plasmid copy number, in agreement with experimental observation.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006288959

PMID: 2950236

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Gramitto, ME.; Froglia, C., 1980: Osservazioni sul potenziale riproduttivo dello scampo (Nephrops norvegicus) in Adriatico. Memorie di Biologia Marina e di Oceanografia, 106: 213-218

Sreenu, Makkena; Kumar, N. Rahul, 2006: Foreign bodies in dogs and a cat. The present paper reports about different foreign bodies in dogs and a cat collected from different parts of the body with treatment.

Abbott, E. V., 1929: A new disease of potato. A quotation from a report of a new rust occurring on potatoes in Canta. It has been recognized as a new species, Aecidium cantensis, by J. C. Arthur, but no description is here given.

Fink, B.; Eckes, J.; Bassenge, E., 2001: Evidence for lipoprotein-induced cellular formation of reactive oxygen species. Pfluegers Archiv European Journal of Physiology 441(6 Supplement): R279

Pahl M.V.; Vaziri N.D.; Khamiseh G.; Oveisi F., 1988: Intestinal absorption of vitamins c and e in experimental azotemia. Clinical Research 36(6): 859A

Herberman, Ronald B., 1992: Cytotoxic activities of lymphocytes. (see Book Title) [Author] Gallin, J I , I M Goldstein and R Snyderman (Ed ) Inflammation: Basic principles and clinical correlates, Second edition xix+1186p Raven Press: New York, New York, USA Illus ISBN 0-88167-880-5 : 791-807

Woods, J. J., 1937: Progress report of the Department of Agriculture Dominion Experimental Station, Saanichton, B.C. Wheat In comparative tests, Sun was the highest yielding winter wheat and Marquis the best spring wheat. A cross made at Ottawa between Dawson's Golden Chaff and Ridit, designated 1968-18L, outyields the former parent and does not shatter at...

Saxena, Vp; Thakur, Rs; Pradhan, K., 1977: Effect of energy protein ratios on the growth and feed efficiency of commercial broilers in summer months. Poultry adviser: 10 (2) 49-54

Burian, J., 1966: The intensity of production, efficiency, and productivity of labour. Agriculture is one of the industries of decisive importance for the stability of the whole national economy of Czechoslovakia. The development of the specialization of production in agriculture greatly affects this stabilization and its introducti...

Dingle, J.T.; Dingle, T.T., 1980: The site of cartilage matrix degradation. 1. A 'cavity' organ culture method was developed, which allowed the insertion within bovine nasal cartilage of 10 microliter of 35S-labelled proteoglycan aggregate. 2. Stimulation of the tissue with retinol or catabolin led to degradatio...