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Regulation of levels of specific sertoli cell messenger rna species by vitamin a


, : Regulation of levels of specific sertoli cell messenger rna species by vitamin a. Developmental Biology 121(2): 316-324

The regulation of Sertoli cell transferrin and sulfated glycoprotein-2 (SGP-2) mRNA levels by vitamin A was studied in vitamin A deficient rats. Vitamin A deficiency differentially affected the levels of these mRNAs. Sertoli cells from vitamn A deficient rats contained 3-fold less transferrin mRNA and 1.8-fold more SGP-2 mRNA than Sertoli cells from normal rats. Vitamin A deficiency did not result in changes in the amount of transferrin mRNA per liver cell. When vitamin A deficient rats were fed a retinol supplemented diet, Sertoli cell transferrin and SGP-2 mRNA levels returned to normal. In contrast, dietary retinoic acid did not support recovery of the levels of the two mRNAs. The regulation of transferrin and SGP-2 mRNA levels was further examined in vitamin A deficient rats that had received subcapsular testicular injections of vitamin A and in vitamin A supplemented cultures of Sertoli cells from vitamin A deficient rats. Under these conditions, retinol and retinoic acid both stimulated transferrin mRNA levels but did not affect SGP-2 mRNA levels. Retinol did not inhibit the turnover of transferrin mRNA in Sertoli cell cultures suggesting that the stimulation of transferrin mRNA levels by retinol is due to increased transcription of the transferrin gene. A mathematical correlation between the Sertoli cell transferrin mRNA levels and the weight of the testes or the number of germinal cells was observed.

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Hugly, S.; Griswold, M., 1987: Regulation of levels of specific Sertoli cell mRNAs by vitamin A. The regulation of Sertoli cell transferrin and sulfated glycoprotein-2 (SGP-2) mRNA levels by vitamin A was studied in vitamin A deficient rats. Vitamin A deficiency differentially affected the levels of these mRNAs. Sertoli cells from vitamin A d...

Hugly, S.; Griswold, M., 1987: Regulation of levels of specific Sertoli cell mRNAs by vitamin A. The regulation of Sertoli cell transferrin and sulfated glycoprotein-2 (SGP-2) mRNA levels by vitamin A was studied in vitamin A deficient rats. Vitamin A deficiency differentially affected the levels of these mRNAs. Sertoli cells from vitamin A d...

Pineau C.; Sharpe R.M.; Saunders P.T.K.; Gerard N.; Jegou B., 1990: Regulation of sertoli cell inhibin production and of inhibin alpha subunit messenger rna levels by specific germ cell types. To elucidate the endocrine and paracrine regulation of testicular inhibin production, the effects of follicle-stimulating hormone (FSH), (Bu)2cAMP, germ cells (either crude or enriched preparations) and germ cell-conditioned media on inhibin produ...

Stallard B.J.; Griswold M.D., 1990: Germ cell regulation of sertoli cell transferrin messenger rna levels. Sertoli cells cultured in the presence of germ cells responded by increasing the level of transferrin mRNA 3-fold as determined by solution hybridization and Northern blot analysis. In contrast, the steady state levels of other mRNAs, including su...

Roberts, K.P.; Awoniyi, C.A.; Santulli, R.; Zirkin, B.R., 1991: Regulation of Sertoli cell transferrin and sulfated glycoprotein-2 messenger ribonucleic acid levels during the restoration of spermatogenesis in the adult hypophysectomized rat. The purpose of this study was to determine whether the levels of sulfated glycoprotein-2 (SGP-2) and/or transferrin mRNA in Sertoli cells responds to testosterone in the adult rat testis and, if so, to distinguish between the effects of testostero...

Dabaja, A.A.; Mielnik, A.; Robinson, B.D.; Wosnitzer, M.S.; Schlegel, P.N.; Paduch, D.A., 2015: Possible germ cell-Sertoli cell interactions are critical for establishing appropriate expression levels for the Sertoli cell-specific MicroRNA, miR-202-5p, in human testis. To examine human microRNA expression in fertile men and subsequently to compare expression patterns of miRNAs in fertile and infertile men, specifically men with Sertoli Cell Only (SCO) histopathology. Testicular tissues from men with azoospermia...

L.M.gueresse-Battistoni, B.; Morera, A.M.; Benahmed, M., 1995: In vitro regulation of rat Sertoli cell inhibin messenger RNA levels by transforming growth factor-beta 1 and tumour necrosis factor alpha. In the present study, we examined the in vitro regulation of 20-day-old rat Sertoli cell inhibin alpha- and beta B-subunits mRNA levels by transforming growth factor-beta 1 (TGF-beta 1) and tumour necrosis factor alpha (TNF alpha), two factors pro...

Morales C.; Hugly S.; Griswold M.D., 1987: Stage dependent levels of specific messenger rna transcripts in sertoli cells. Localization and stage-dependent levels of transferrin and sulfated glycoprotein-2 (SGP-2) mRNAs were examined in rat testes by in situ and soluble hybridization of mRNA with a single-stranded RNA probe prepared with the SP65 vector. Biotinylated...

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