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Regulatory function of elongation factor g in bacillus subtilis sporulation



Regulatory function of elongation factor g in bacillus subtilis sporulation



Journal of Science of the Hiroshima University Series B Division 1 (Zoology) 30(2): 281-292



Strain FUS426 (fusr spots) isolated spontaneously from B. subtilis 168 was a temperature-sensitive sporulation mutant having an altered elongation factor G. The altered elongation factor G of FUS426 had a more basic isoelectric point than that of the parental strain. EM showed that the sporulation of FUS426 cultivated at 42.degree. C was blocked at stage O-I. The temperature-sensitive period of sporulation of FUS426 was the early stage of sporulation (T1-T2; the hours after the end point of the logarithmic growth are expressed as T1, T2, etc.). Analyses of membrane proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis during sporulation revealed that T2.5-cells of FUS426 did not synthesize 2 membrane proteins (MP105 and MP55) at the nonpermissive temperature, while those of the parental strain synthesized MP105 and MP55. Sporulation-positive revertants (fuss spo+) of FUS426 synthesized MP105 and MP55. MP105 and MP55 were newly synthesized at the early stage of sporulation.

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Accession: 006292087

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