Reinvestigation of purification of phenol oxidase ec from larvae of house fly musca domestica vicina

Yonekura, M.; Shimoda, T.; Funatsu, M.

Agricultural and Biological Chemistry 45(1): 101-104


ISSN/ISBN: 0002-1369
Accession: 006292940

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Phenol oxidase (diphenol oxidase) was purified from an aqueous extract of the larvae of the housefly M. d. vicina Maquart by a procedure involving affinity chromatography on a Sepharose 4B derivative of p-aminobenzoic acid and ethylene diamine, Sepharose 4B and Sephadex G-200 gel filtrations. The purified phenol oxidase was homogeneous on polyacrylamide gel electrophoresis. Using this improved method, phenol oxidase was recovered in greater yield and specific activity when compared with a previous purification procedure.