EurekaMag
+ Translate
+ Most Popular
Spermatogenic and steroidogenic impairment after chromium treatment in rats
Prediction of enthalpy of formation based on refined crystal structures of multisite compounds; Part 1, Theories and examples
Intrastromal photorefractive keratectomy for myopia by Nd:YLF picosecond laser
Teachers' beliefs about the role of prior language knowledge in learning and how these influence teaching practices
Classification and genesis of submarine iron-manganese deposits
Insecta a Cl. G. N. Potanin in China et in Mongolia novissime lecta. XVII. Hemiptera-Heteroptera
On the occurrence of the splendid genus Machaerothrix Haupt in Tsushima, with some remarks on the genus (Hymenoptera, Pompilidae)
Enamel craze lines
Gynecologic examination of the child and the adolescent
Periosteal osteoclasts, skeletal development and ageing
Fibrodysplasia ossificans progressiva in a Nigerian: a case report
The chronically ill patient with anorexia nervosa development, phenomenology, and therapeutic considerations
Kummelonautilus taiwanum sp. nov. (Nautilida) from the early Miocene Shihmen Formation, Chungliao, Nantou, west-central Taiwan
Efficacy of Droncit against Dipylidium caninum in dogs
Pierids of the genus Euchloe Klots (Lepidoptera, Pieridae) from Siberia and the Far East.
C5A anaphylatoxin and its seven transmembrane-segment receptor
Failure to practice evidence-based medicine: why do physicians not treat patients with heart failure with angiotensin-converting enzyme inhibitors?
Empirical comparisons of proportional hazards regression, Poisson regression, and logistic regression in the analysis of occupational cohort data
Pollination of flowers of sweet and sour cherries
Isopodi terrestri raccolti nell' Estremo Oriente
Morphometric observations on Dipylidium caninum from dogs and cats in Austria
Chelation effectiveness of zinc proteinates demonstrated
Alopecia--a psychosomatic disease picture? I. Review of the literature
Monitoring equity in health and healthcare: a conceptual framework
Hemodynamic evaluation of the Biocor heterologous prosthesis

Relationship between cellular calcium and prostaglandin synthesis in cultured vascular smooth muscle cells


Relationship between cellular calcium and prostaglandin synthesis in cultured vascular smooth muscle cells



Prostaglandins 32(3): 457-478



ISSN/ISBN: 0090-6980

PMID: 3097761

DOI: 10.1016/0090-6980(86)90012-2

We have investigated the effects of extracellular and intracellular Ca deficits and of pharmacologic agents thought to inhibit Ca influx or intracellular Ca mobilization on vasopressin-evoked changes of cytosolic Ca2+ levels and PG synthesis in cultured rat mesenteric arterial vascular smooth muscle cells. Vasopressin rapidly increased cytosolic Ca2+ as well as PG synthesis. The increase of cytosolic Ca2+ and the rate of PG synthesis were both maximal within the first minute of incubation. An extracellular Ca deficit of short duration partially inhibited both vasopressin-evoked PG synthesis and the increase of cytosolic Ca2+ by 40 to 60%. Two procedures which deplete cells of some of their intracellular Ca, namely a 30 min incubation in EGTA-supplemented, Ca-lacking media, or a 1 min incubation with ionophore A23817 in Ca-deficient media, decreased PG synthesis by 65% to 100%. The addition of extracellular Ca to Ca-depleted cells restored the ability of vasopressin to stimulate PG synthesis. Two Ca channel antagonists, nifedipine or cinnarizine, had no effect on either vasopressin-evoked PG synthesis or increased cytosolic Ca2+, whereas TMB-8 (10 .mu.M), a putative inhibitor of intracellular Ca mobilization, decreased PG synthesis by 75% by inhibiting acylhydrolase as well as cyclo-oxygenase activities, but had no effect on basal or vasopressin-evoked increase of cytosolic Ca2+, documenting that its inhibitory effect was not a consequence of decreased cytosolic Ca2+. These results demonstrate that decreased cellular Ca levels are associated with decreased cytosolic Ca2+ levels and PG synthesis, and support the hypothesis of a link between, on the one hand, cellular Ca and/or cytosolic Ca2+ and on the other hand, PG synthesis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 006296154

Download citation: RISBibTeXText

Related references

Simultaneous measurements of cytosolic free calcium level and prostaglandin synthesis reveal a correlation between them in perfused monolayer of cultured rat vascular smooth muscle cells: effects of bradykinin and angiotensin II. Tohoku Journal of Experimental Medicine 165(3): 183-192, 1991

Effect of in vitro aging on prostaglandin synthesis in cultured rat vascular smooth muscle cells. Agents and Actions. Supplements 22: 49-54, 1987

Effect of sulindac on prostaglandin synthesis in human and in cultured rat renal and vascular smooth muscle cells. Tohoku Journal of Experimental Medicine 166(1): 185-200, 1992

Stimulation of prostacyclin synthesis by thromboxane A2-like prostaglandin endoperoxide analogues in cultured vascular smooth muscle cells. Biochemical and Biophysical Research Communications 123(1): 21-26, 1984

Role of calcium in angiotensin II-induced prostaglandin release and DNA synthesis in rat vascular smooth muscle cells. Journal of Cardiovascular Pharmacology 27(2): 195-200, 1996

Enhancement of endotoxin-induced prostaglandin synthesis by elevation of glucose concentration in primary cultured rat vascular smooth muscle cells. Yakhak Hoeji 41(6): 782-788, 1997

Effects of calcium-antagonists and calmodulin inhibitors on DNA synthesis in cultured rat vascular smooth muscle cells. Endocrinologia Japonica 34(2): 313-318, 1987

Role of calcium-independent phospholipase A2beta in high glucose-induced activation of RhoA, Rho kinase, and CPI-17 in cultured vascular smooth muscle cells and vascular smooth muscle hypercontractility in diabetic animals. Journal of Biological Chemistry 285(12): 8628-8638, 2010

Non linear relationship between alpha 1 adrenergic receptor occupancy and calcium 45 efflux in cultured vascular smooth muscle cells. Clinical Research 33(2 Part 1): 360A, 1985

Effects of calcium antagonists on vasoconstrictor hormone evoked prostacyclin synthesis in cultured vascular smooth muscle cells. Kidney International 29(1): 260, 1986

Nonlinear relationship between alpha 1-adrenergic receptor occupancy and norepinephrine-stimulated calcium flux in cultured vascular smooth muscle cells. Molecular Pharmacology 27(5): 517-524, 1985

Vasoconstrictor-evoked prostaglandin synthesis in cultured vascular smooth muscle. American Journal of Physiology 245(3): C278-C282, 1983

Expression of smooth muscle-specific a-isoactin in cultured vascular smooth muscle cells: relationship between growth and cytodifferentiation. The Journal of Cell Biology 102: 3-52, 1986

Expression of Smooth Muscle-Specific -Isoactin in Cultured Vascular Smooth Muscle Cells: Relationship between Growth and Cytodifferentiation. The Journal of Cell Biology 102(2): 343-352, 1986

Expression of smooth muscle-specific alpha-isoactin in cultured vascular smooth muscle cells: relationship between growth and cytodifferentiation. Journal of Cell Biology 102(2): 343-352, 1986