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Relationship between loss of viability and cell lysis in sodium chloride osmotic shock in escherichia coli



Relationship between loss of viability and cell lysis in sodium chloride osmotic shock in escherichia coli



Agricultural and Biological Chemistry 41(2): 251-256



E. coli cells at the log growth phase lost colony-forming ability when treated in 0.01 M Tris buffer containing 0.15 M NaCl and then diluted with 0.01 M Tris buffer. The NaCl-treated cells did not lose colony-forming ability when they were diluted with 0.01 M Tris buffer containing 0.15 M NaCl. When the concentration of Tris buffer for dilution was raised to 0.15 M, the decrease in survival was effectively prevented. Lysis of NaCl-treated cells was observed during incubation in 0.01 M Tris buffer. This cell lysis was inhibited by the presence of Mg2+, Ca2+ or Mn2+ in the dilution buffer and by incubation at 0.degree. C. The cell lysis seemed to be unrelated to the loss of viability, since viability decreased rapidly when the NaCl-treated cells were diluted with 0.01 M Tris buffer at 0.degree. C, where the cell lysis was not observed. A polymyxin B resistant mutant was more sensitive to NaCl-treatment but was more resistant to sucrose-osmotic shock than its parent strain. The saline sensitive phenomenon probably involves osmotic shock of the NaCl-treated cells. Thus, this phenomenon NaCl-osmotic shock.

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