EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Relationship between loss of viability and cell lysis in sodium chloride osmotic shock in escherichia coli


, : Relationship between loss of viability and cell lysis in sodium chloride osmotic shock in escherichia coli. Agricultural and Biological Chemistry 41(2): 251-256

E. coli cells at the log growth phase lost colony-forming ability when treated in 0.01 M Tris buffer containing 0.15 M NaCl and then diluted with 0.01 M Tris buffer. The NaCl-treated cells did not lose colony-forming ability when they were diluted with 0.01 M Tris buffer containing 0.15 M NaCl. When the concentration of Tris buffer for dilution was raised to 0.15 M, the decrease in survival was effectively prevented. Lysis of NaCl-treated cells was observed during incubation in 0.01 M Tris buffer. This cell lysis was inhibited by the presence of Mg2+, Ca2+ or Mn2+ in the dilution buffer and by incubation at 0.degree. C. The cell lysis seemed to be unrelated to the loss of viability, since viability decreased rapidly when the NaCl-treated cells were diluted with 0.01 M Tris buffer at 0.degree. C, where the cell lysis was not observed. A polymyxin B resistant mutant was more sensitive to NaCl-treatment but was more resistant to sucrose-osmotic shock than its parent strain. The saline sensitive phenomenon probably involves osmotic shock of the NaCl-treated cells. Thus, this phenomenon NaCl-osmotic shock.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006297520

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Datta, NC.; Maity, NC., 1986: Olfactory apparatus of Sillago sihama (Forskal) and Sillaginopsis panijus (Hamilton-Buchanan) (Perciformes-Sillaginidae). Matsya. 1985 ; 5-11 No. 11

Miller S.E.; Hadfield M.G., 1990: Developmental arrest during larval life and life span extension in a marine mollusc. The length of larval life in the nudibranch Phestilla sibogae is determined by a chance encounter with a specific metamorphic stimulus associated with the post-larval benthic habitat. A developmental hiatrus begins at the onset of larval metamorph...

Zackson H.J.; Chan L.; Montague D.; Cole R.P., 1986: Clinical significance of a reduced pulmonary carbon monoxide diffusing capacity to vital capacity ratio. American Review of Respiratory Disease 133(4 SUPPL): A130

Gorman, C.; Leandro, N. I.; Isenberg, D., 2004: Does B cell depletion have a role to play in the treatment of systemic lupus erythematosus?. B cell dysfunction and pathogenic autoantibody formation are thought to be critical in the pathogenesis of systemic lupus erythematosus (SLE). In this review we will summarize the results of attempts to utilize B cell depletion, based on the use o...

Brown M.F.; Dodd S.W.; Henriksson U.; Salmon A.; Soderman O., 1989: Molecular dynamics of phospholipid bilayers in the liquid crystalline state from deuterium nmr spectroscopy. Biophysical Journal 55(2 PART 2): 102A

Borcea, I., 1928: Nouvelles observations sur la faune cotiere du littoral Roumain de la Mer Noire. Annales Universite Jassy, 15: 286-298

Silver Marshall L., 1986: Simplified analysis techniques. Pages 631-697:

Matthews W.S., 1986: Neurologic correlates of osteogenesis imperfecta. The neurologic status of ten patients with osteogenesis imperfecta (OI) was evaluated. Four patients with mild OI (type I) had normal neurologic findings and normal computed tomographic scans. Three of these four patients had macrocephaly. All six...

Hardisson C., 1985: Beta lactam resistance and characterization of plasmid mediated beta lactamases in clinical isolates of serratia. The in vitro activity of eleven beta-lactam antibiotics against one hundred clinical isolates of Serratia was studied. All the isolates were susceptible to cefuroxime, ceftriaxone, moxalactam and N-formimidoyl thienamycin; more than 50% were susce...

Vahid, Y., 1979: Production, consumption and level of animal proteins in developing countries. Allattenyesztes = Animal breeding8(4): 335-341