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Relationship between loss of viability and cell lysis in sodium chloride osmotic shock in escherichia coli

Agricultural and Biological Chemistry 41(2): 251-256

Relationship between loss of viability and cell lysis in sodium chloride osmotic shock in escherichia coli

E. coli cells at the log growth phase lost colony-forming ability when treated in 0.01 M Tris buffer containing 0.15 M NaCl and then diluted with 0.01 M Tris buffer. The NaCl-treated cells did not lose colony-forming ability when they were diluted with 0.01 M Tris buffer containing 0.15 M NaCl. When the concentration of Tris buffer for dilution was raised to 0.15 M, the decrease in survival was effectively prevented. Lysis of NaCl-treated cells was observed during incubation in 0.01 M Tris buffer. This cell lysis was inhibited by the presence of Mg2+, Ca2+ or Mn2+ in the dilution buffer and by incubation at C. The cell lysis seemed to be unrelated to the loss of viability, since viability decreased rapidly when the NaCl-treated cells were diluted with 0.01 M Tris buffer at C, where the cell lysis was not observed. A polymyxin B resistant mutant was more sensitive to NaCl-treatment but was more resistant to sucrose-osmotic shock than its parent strain. The saline sensitive phenomenon probably involves osmotic shock of the NaCl-treated cells. Thus, this phenomenon NaCl-osmotic shock.

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Accession: 006297520

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