EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Relationship between Moloney murine leukemia and sarcoma virus RNAs: purification and hybridization map of complementary DNAs from defined regions of Moloney murine sarcoma virus 124


Relationship between Moloney murine leukemia and sarcoma virus RNAs: purification and hybridization map of complementary DNAs from defined regions of Moloney murine sarcoma virus 124



Journal of Virology 23(3): 524-532



ISSN/ISBN: 0022-538X

PMID: 197259

Complementary DNA (cDNA) specific for various regions of the Moloney murine sarcoma virus (MSV) 124 RNA genome were prepared by cross-hybridization techniques. A cDNA specific for the first 1000 nucleotides adjacent to the RNA 3' end (cDNA 3') was prepared and was complementary to the 3'-terminal 1000 nucleotides of a related Moloney murine leukemia virus (MLV) genome. A cDNA complementary to the MSV-specific portion of the MSV 124 genome was prepared. This cDNA did not anneal to Moloney MLV RNA but annealed to a portion of the viral RNA of about 1500-1800 nucleotides in length, located 1000 nucleotides from the 3' end MSV RNA. A cDNA common to the genome of MSV and MLV was also obtained and it annealed to the 5'-terminal two thirds, and to the 3'-terminal 1000 nucleotides of the MSV RNA genome. This cDNA also annealed to the RNA from MLV and mainly to the 5'-terminal half of the MLV genome. The 6-kilobase Moloney MSV 124 RNA genome has a sequence arrangement that includes a 3' portion of about 1000 nucleotides, which is also present at the 3' terminus of MLV; an MSV-specific region, not shared with MLV, which extends between 1000-2500 nucleotides from the 3' terminus; and a 2nd common region, again shared with MLV, which extends from 2500 nucleotides to the 5' terminus. This 2nd common region also appears to be located in the 5' half of the 10-kilobase MLV genome. Experiments in which a large excess of cold MLV cDNA was annealed to 3H-labeled poly(A)-containing fragments of MSV RNA gave results consistent with this arrangement of the MSV genome.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 006297711

Download citation: RISBibTeXText

Related references

Suppression of murine retrovirus polypeptide termination: effect of amber suppressor tRNA on the cell-free translation of Rauscher murine leukemia virus, Moloney murine leukemia virus, and Moloney murine sarcoma virus 124 RNA. Journal of Virology 34(2): 464-473, 1980

Restriction of Moloney murine leukemia virus replication in Moloney murine sarcoma virus-infected cells - Restriction de la réplication du virus de la leucémie de Moloney dans les cellules infectées par le virus du sarcome murin de Moloney. Virology (New York) 132(2): 377-389, 1984

Translation of moloney murine leukemia virus and moloney murine sarcoma virus rna in nuclease treated reticulocyte extracts enhancement of the gag pol poly peptide with yeast suppressor transfer rna. Cell 13(1): 189-200, 1978

Significant differences in integration sites of Moloney murine leukemia virus/Moloney murine sarcoma virus retroviral vector carrying recombinant coagulation factor IX in two human cell lines. Biotechnology Letters 37(5): 991-1001, 2015

Vertical transmission of murine leukemia virus moloney and murine sarcoma virus moloney and related problems. Yamamoto, Tadashi And Haruo Sugano (Ed ) Gann Monograph on Cancer Research, No 12 Experimental Leukemogenesis Symposium Tokyo, Japan Nov 13-14, 1970 Viii+321p Illus University Park Press: Baltimore, Md , U S A ; Tokyo, Japan 11-31, 1972

Restriction of Moloney murine leukemia virus replication in Moloney murine sarcoma virus-infected cells. Virology 132(2): 377-389, 1984

Resistance to Moloney murine sarcoma virus (M-MuSV) tumor induction is associated with natural antibody production to "endogenous" Moloney leukemia virus (M-MuLV) in BALB/Mo mice. Tumori 67(6): 511-520, 1981

Sequence homology between Moloney murine sarcoma virus and Moloney leukemia virus RNA. Journal of Virology 19(1): 82-89, 1976

Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus. Journal of Virology 34(1): 200-212, 1980

Purification and characterization of the DNA polymerase and RNase H activities in Moloney murine sarcoma-leukemia virus. Journal of Virology 15(4): 785-797, 1975

Studies on mouse Moloney virus induced tumours: I. The detection of p30 as a cytotoxic target on murine Moloney leukaemic spleen cells, and on an in vitro Moloney sarcoma line by antibody mediated cytotoxicity. British Journal of Cancer 31(5): 499-512, 1975

Electrophoretic comparisons of the poly peptide composition of the murine and feline leukemia virus pseudotypes of moloney murine sarcoma virus. Abstracts of the Annual Meeting of the American Society for Microbiology 74: 238, 1974

T cell tolerance in moloney-murine leukemia virus (M-MuLV) carrier mice: low cytotoxic T lymphocyte precursor frequency and absence of suppressor T cells in carrier mice with Moloney-murine sarcoma (M-MSV)-induced tumors. Journal of Immunology 128(2): 774-779, 1982

Cleavage of rauscher murine leukemia virus pr 65g a g by the moloney murine leukemia virus proteolytic activity produces rauscher murine leukemia virus specific but not moloney murine leukemia virus specific 40000 dalton intermediate poly peptide. Journal of General Virology 54(1): 33-38, 1981

Temperature sensitive moloney murine sarcoma virus a deletion mutant of wild type murine sarcoma virus coding for an 85 kilodalton gag src poly protein. Abstracts of the Annual Meeting of the American Society for Microbiology 82: T119, 1982