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Relationship between the amounts of epstein barr virus dna and epstein barr virus nuclear antigen per cell clonability and tumorigenicity in 2 epstein barr virus negative lymphoma lines and their epstein barr virus converted sublines



Relationship between the amounts of epstein barr virus dna and epstein barr virus nuclear antigen per cell clonability and tumorigenicity in 2 epstein barr virus negative lymphoma lines and their epstein barr virus converted sublines



International Journal of Cancer 31(2): 163-170



The effect of Epstein-Barr Virus(EBV)-conversion of 2 EBV-negative human lymphoma lines (Ramos and BJAB) on agarose clonability and tumorigenicity in nude mice was explored. The cloning frequency was increased in all 9 sublines investigated, between 1.1 and 4.9 times compared to the original parental lines. Tumorigenicity was increased in 1 of 2 BJAB-derived, EBV-positive lines and 3 of 6 Ramos-derived lines, while it was decreased in 3 others. A strong positive correlation between the number of genomes/cell and the amount of Epstein-Barr nuclear antigen [EBNA]/cell was detected, and in 7 of 9 converted lines the cloning frequency also correlated to the number of EBV genomes. No relation was established between the number of EBV genomes/cell, the amount of EBNA/cell and tumorigenicity.

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