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Release of neuropeptide y like immunoreactive material from primary cultures of chromaffin cells prepared from bovine adrenal medulla



Release of neuropeptide y like immunoreactive material from primary cultures of chromaffin cells prepared from bovine adrenal medulla



Neuropharmacology 24(7): 693-695



In primary cultures of bovine chromaffin cells, the cell contents of [neuropeptide Y] NPY immunoreactive material were stable for about 4 days. When these cells were transferred to the Locke's solution from the culture medium, NPY immunoreactive material was released spontaneously. Addition of K (45 mM) resulted in a 3-fold increase in the rate of NPY release in a Ca-dependent fashion. NPY immunoreactivity in chromaffin cells is composed of NPY, a lesser immunoreactive peptide and 2 very minor immunoreactivities. The same immunoreactive peptides were released by 45 mM K.

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Accession: 006306143

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