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Reliability of molecular weight determination of proteins by poly acrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate/



Reliability of molecular weight determination of proteins by poly acrylamide gradient gel electrophoresis in the presence of sodium dodecyl sulfate/



Analytical Biochemistry 85(1): 114-125



Proteins (34) with MW between 1.3 .times. 104-9.5 .times. 105 and 7 polypeptide chains of some of these proteins were incubated in sodium dodecyl sulfate and submitted to electrophoresis in a polyacrylamide gradient gel (3-30%; relative percentage of bisacrylamide: 8.4%). Analysis of the results confirmed the reliability of the relationship: log (MW) = a log (T) + b, where T is the polyacrylamide concentration reached by a protein, a is the slope and b is the Y intercept of the regression line. The mean deviation between the expected and the observed MW was .+-. 5.5%. MW of unreduced and reduced proteins can be estimated with the same accuracy, and MW of glycoproteins can also be determined with the same accuracy as other proteins.

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Accession: 006307050

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PMID: 629376


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