Repair in vitro of nitrate reductase ec deficient tobacco nicotiana tabacum mutants cnx a by molybdate and by molybdenum cofactor

Mendel, R.R.; Mueller, A.J.

Planta (Heidelberg) 163(3): 370-375


ISSN/ISBN: 0032-0935
Accession: 006312244

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Two nitrate reductase-deficient mutant cell lines (CnxA68/2, CnxA101) of N. tabacum are repairable under in-vitro conditions by molybdate or by preparations of active Mo cofactor of homologous or heterologous origin, thereby yielding about 20% and 80%, respectively, of the corresponding wild-type NADH-nitrate reductase (EC activity. In-vitro repair of nitrate reductase activity is dependent on sulfhydryl-group protecting reagents and EDTA in the extraction medium, the N source in the growth medium and the age of the cells. The cnxA gene controls the insertion of Mo into the Mo cofactor. They are consistent with the idea of 2 interlinked pathways for the metabolic processing of Mo acquisition, one involving the synthesis of the structural moiety of the Mo cofactor and the other involving processing of the molybdate anion.