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Repair of methyl methanesulfonate induced dna double strand breaks in ha ploid cells of saccharomyces cerevisiae which requires the presence of a duplicate genome



Repair of methyl methanesulfonate induced dna double strand breaks in ha ploid cells of saccharomyces cerevisiae which requires the presence of a duplicate genome



Molecular & General Genetics 167(3): 279-286



The formation and repair of double-strand breaks induced in DNA by MMS [methyl methanesulfonate] was studied in haploid wild type and MMS-sensitive rad6 mutant strains of S. cerevisiae with the use of the neutral and alkaline sucrose sedimentation technique. A similar decrease in average MW of double-stranded DNA from 5-6 .times. 108 to 1-0.7 .times. 108 daltons was observed following treatment with 0.5% MMS in wild type and mutant strains. Incubation of cells after MMS treatment in a fresh drug-free growing medium resulted in repair of double-strand breaks in the wild type strain, but only in the exponential phase of growth. No repair of double-strand breaks was found when cells of the wild type strain were synchronized in G-1 phase by treatment with .alpha. factor, although DNA single-strand breaks were still efficiently repaired. Mutant rad6 which has a very low ability to repair MMS-induced single-strand breaks, did not repair double-strand breaks regardless of the phase of growth. Repair of double-strand breaks probably requires the ability for single-strand breaks repair, and rejoining of double-strand breaks apparently requires the availability of 2 homologous DNA molecules, strongly supporting the recombinational model of DNA repair.

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