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Repairable and unrepairable dna strand breaks induced by decay of tritium and iodine 125 incorporated into dna of mammalian cells






Radiation & Environmental Biophysics 18(4): 239-248

Repairable and unrepairable dna strand breaks induced by decay of tritium and iodine 125 incorporated into dna of mammalian cells

Chinese hamster fibroblast cells (Cl:1) were labeled with 3H-thymidine or 125iododeoxyuridine for 18 h. After 3 h in non-radioactive medium they were stored at 0.degree. C up to 6 h. The number of DNA strand breaks observed after the labeling period (37.degree. C) or after treatment at 0.degree. C was determined using the DNA-unwinding technique. 125I-decays in DNA were significantly more efficient than 3H-decays in introducing unrepairable DNA strand breaks during the labeling period. After 21 h at 37.degree. C, 32% of 125I-induced and 3% of 3H-induced DNA strand breaks were unrepaired. The effects of 125I- or 3H-disintegrations in DNA were compared in 3 different ways. Effects were 7-12 times more pronounced for 125I-decays. For 125I-labeled cells 3-4 DNA strand breaks were found per decay. The corresponding value for 3H-labeled cells was 2.

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Accession: 006312686



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