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Repairable and unrepairable dna strand breaks induced by decay of tritium and iodine 125 incorporated into dna of mammalian cells



Repairable and unrepairable dna strand breaks induced by decay of tritium and iodine 125 incorporated into dna of mammalian cells



Radiation & Environmental Biophysics 18(4): 239-248



Chinese hamster fibroblast cells (Cl:1) were labeled with 3H-thymidine or 125iododeoxyuridine for 18 h. After 3 h in non-radioactive medium they were stored at 0.degree. C up to 6 h. The number of DNA strand breaks observed after the labeling period (37.degree. C) or after treatment at 0.degree. C was determined using the DNA-unwinding technique. 125I-decays in DNA were significantly more efficient than 3H-decays in introducing unrepairable DNA strand breaks during the labeling period. After 21 h at 37.degree. C, 32% of 125I-induced and 3% of 3H-induced DNA strand breaks were unrepaired. The effects of 125I- or 3H-disintegrations in DNA were compared in 3 different ways. Effects were 7-12 times more pronounced for 125I-decays. For 125I-labeled cells 3-4 DNA strand breaks were found per decay. The corresponding value for 3H-labeled cells was 2.

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