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Repeated consensus sequence and pseudopromoters in the 4 coordinately regulated tubulin genes of chlamydomonas reinhardi






Molecular & Cellular Biology 4(6): 1115-1124

Repeated consensus sequence and pseudopromoters in the 4 coordinately regulated tubulin genes of chlamydomonas reinhardi

The 5' coding and promoter regions of the 4 coordinately regulated tubulin genes of C. reinhardi were mapped and sequenced. DNA sequencing data shows that the predicted N-terminal amino acid sequences of Chlamydomonas .alpha.- and .beta.-tubulins closely match that of tubulins of other eukaryotes. Within the .alpha.1- and .alpha.2-tubulin gene set and the .beta.1- and .beta.2-tubulin gene set, both nucleotide sequence and intron placement are highly conserved. Transcription initiation sites were located by primer extension analysis at 140, 141, 159 and 132 base pairs upstream of the translation initiator codon for the .alpha.1- .alpha.2-, .beta.1- and .beta.2-tubulin genes, respectively. Among the structures with potential regulatory significance, the most striking is a 16-base-pair consensus sequence [GCTC(G/C)AAGGC(G/T)(G/C)-(C/A)(C/A)G] which is found in multiple copies immediately upstream of the TATA box in each of the 4 genes. An unexpected discovery is the presence of pseudopromoter regions in 2 of the transcribed tubulin genes. One pseudopromoter region is located 400 base pairs upstream of the authentic .alpha.2-tubulin gene promoter, whereas the other is located within the transcribed 5' noncoding region of the .beta.1-tubulin gene.

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Accession: 006312896



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