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Repression of inducible enzyme synthesis in a mutant of Escherichia coli K 12 deleted for the ptsH gene



Repression of inducible enzyme synthesis in a mutant of Escherichia coli K 12 deleted for the ptsH gene



Molecular & General Genetics 153(2): 185-190



The genome of .lambda. phage with thermosensitive repressor was inserted into the pts region of the E. coli chromosome. This lysogenic culture possessed the PTS [phosphoenolpyruvate-dependent phosphotransferase system] phenotype at 30.degree. C. A mutant strain with a deletion covering the ptsH gene was isolated after a prophage curing procedure. The deletion nature of the pts mutation was confirmed in genetical and biochemical experiments. The deletion covered a small fragment of the bacterial genome not extending in the ptsI and lig genes. The isolated .DELTA.ptsH mutant possessed all characteristics of known pts mutants:pleiotropical disturbances of transport and utilization of a number of carbohydrates, repression of the enzyme inducible synthesis and resistance to glucose catabolite repression. These and other data showed that the phosphorylated form of the heat-stable protein HPr is involved (directly or indirectly) in activation of the DNA transcription process.

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Accession: 006315016

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PMID: 329116


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