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Repression of insulin gene expression by adenovirus type 5 E1a proteins



Repression of insulin gene expression by adenovirus type 5 E1a proteins



Molecular and Cellular Biology 7(3): 1164-1170



Insulin gene transcription relies on enhancer and promoter elements which are active in pancreatic beta cells. We showed that adenovirus type 5 infection of HIT T-15 cells, a transformed hamster beta cell line, represses insulin gene transcription and mRNA levels. Using expression plasmids transiently introduced into HIT T-15 cells, we showed that adenovirus type 5 E1a transcription regulatory proteins repress insulin enhancer-promoter element activity as assayed with a surrogate xanthine-guanine phosphoribosyltransferase gene. We relate E1a repression of the insulin gene to other examples of repression of enhancer-dependent genes by E1a and discuss the possible relationship of this repression to insulin gene regulation.

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Accession: 006315018

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PMID: 2951590

DOI: 10.1128/mcb.7.3.1164


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