+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Repression of insulin gene expression by adenovirus type 5 E1a proteins

Repression of insulin gene expression by adenovirus type 5 E1a proteins

Molecular and Cellular Biology 7(3): 1164-1170

Insulin gene transcription relies on enhancer and promoter elements which are active in pancreatic beta cells. We showed that adenovirus type 5 infection of HIT T-15 cells, a transformed hamster beta cell line, represses insulin gene transcription and mRNA levels. Using expression plasmids transiently introduced into HIT T-15 cells, we showed that adenovirus type 5 E1a transcription regulatory proteins repress insulin enhancer-promoter element activity as assayed with a surrogate xanthine-guanine phosphoribosyltransferase gene. We relate E1a repression of the insulin gene to other examples of repression of enhancer-dependent genes by E1a and discuss the possible relationship of this repression to insulin gene regulation.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 006315018

Download citation: RISBibTeXText

PMID: 2951590

DOI: 10.1128/mcb.7.3.1164

Related references

Repression of cardiac gene expression and reactivation of cardiac growth by adenovirus E1A proteins. Circulation 90(4 Part 2): I195, 1994

Control of cellular gene expression during adenovirus infection: induction and shut-off of dihydrofolate reductase gene expression by adenovirus type 2. Molecular and Cellular Biology 3(5): 819-828, 1983

Induction of gene expression by exon 2 of the major E1A proteins of adenovirus type 5. Journal of Virology 67(12): 6922-6928, 1993

Regulation of viral and cellular gene expression by E1A proteins encoded by the oncogenic adenovirus type 12. Current Topics in Microbiology and Immunology 199: 81, 1995

Proteins containing only half of the coding information of early region 1b of adenovirus are functional in human cells transformed with the herpes simplex virus type 1 thymidine kinase gene and adenovirus type 2 DNA. Journal of Virology 41(2): 423-434, 1982

Repression of cytochrome P-450c gene expression by cotransfection with adenovirus E1a DNA. European Journal of Biochemistry 181(3): 539-544, 1989

Sequence-mediated regulation of adenovirus gene expression by repression of mRNA accumulation. Molecular and Cellular Biology 17(4): 2207-2216, 1997

Viral gene inhibition of class I major histocompatibility antigen expression: not a general mechanism governing the tumorigenicity of adenovirus type 2-, adenovirus type 12-, and simian virus 40-transformed Syrian hamster cells. Journal of Virology 62(8): 2755-2761, 1988

Repression of cyclin D1 expression does not contribute to initiation or maintenance of cell transformation by adenovirus type 5 E1. Journal of Virology 70(11): 7804-7810, 1996

Infectivity and expression of the early adenovirus proteins are important regulators of wild-type and DeltaE1B adenovirus replication in human cells. Oncogene 18(36): 5032-5043, 1999

Expression of adenovirus type 2 late genes in HeLa cells infected with adenovirus type 2 or adenovirus type 2-simian virus 40 hybrid viruses. Journal of Virology 40(2): 610-614, 1981

Repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus. Journal of Virology 57(1): 13-17, 1986

Expression of unselected adenovirus genes in human cells co transformed with the herpes simplex virus type 1 tk gene and adenovirus 2 dna. Cell 21(2): 453-464, 1980

IL-21 imposes a type II EBV gene expression on type III and type I B cells by the repression of C- and activation of LMP-1-promoter. Proceedings of the National Academy of Sciences of the United States of America 107(2): 872-877, 2010

Expression of chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters effect of promoter methylation on gene expression. Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene Series A 257(1): 144, 1984