Repression of the byproduct in enzymatic production of l aspartic acid industrial use of ethanol utilizing microorganisms 2
Yukawa, H.; Nara, T.; Terasawa, M.; Takayama, Y.
Nippon Nogeikagaku Kaishi 59(3): 279-286
Conditions were investigated for a high yield of L-aspartic acid by thermal inactivation of intracellular fumarase. The optimal concentrations were established for inactivation of fumarase without a decrease in aspartase activity. Both L-aspartic acid and CaCl2 protected against thermal inactivation of aspartase activity in intact cells. The residual activity of intracellular fumarase was negligible. Then the cells were incubated at 45.degree. C for 5 h in a mixture consisting of 2(mol) NH3, 750 (mM) CaCl2 and 0.08 (% wt/vol) detergent Tween 20. Treatment for inactivation of intracellular fumarase resulted in stabilization of intracellular aspartase.