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Reproducibility of histamine skin prick test intervariation and intravariation using histamine dihydrochloride 1 5 and 10 milligram per milliliter

Reproducibility of histamine skin prick test intervariation and intravariation using histamine dihydrochloride 1 5 and 10 milligram per milliliter

Allergy (Copenhagen) 40(5): 344-349

The reproducibility of skin prick test using histamine dihydrochloride 1, 5, and 10 mg/ml was tested by 3 nurses in 5 non-atopics in a double-blind trial. The variations day-to-day, within-day, between and for the same tester were calculated. Seventy-five percent of wheal reactions obtained by histamine 1 mg/ml were < 15 mm2. With histamine 5 mg/ml there were only a few wheals < 15 mm2 and none at all with histamine 10 mg/ml. The mean coefficient of variation of wheals > 15 mm2 was between 20-30%, in contrast to figures between 30-60% with wheals < 15 mm2. No significant day-to-day or within-day variation was shown concerning histamine wheal areas. Histamine dihydrochloride 10 mg/ml should replace histamine dihydrochloride 1 mg/ml as the positive reference in routine skin prick tests and biological standardization.

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