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Reproducibility of measurements of estrogen receptor concentration in breast cancer

Reproducibility of measurements of estrogen receptor concentration in breast cancer

British Journal of Cancer 36(3): 355-361

The reproducibility of measurements of estrogen receptor activity was examined in multiple specimens from a rabbit uterus, a rat mammary tumor and human breast tumors. The relationship between receptor concentration and tumor histology was also investigated in 11 large primary tumors. In the animal tissues, receptor measurements were relatively reproducible (coefficient of variance [cv]: wet weight basis 16-17%, protein basis 16-21%) but in human breast tumors receptor activity varied considerably (c.v.: wet weight basis, 22-125%; protein basis, 28-72%). In addition to these variations in receptor activity within tumors, there was a difference between tumors, as demonstrated by an analysis of variance (P < 0.01). In the 11 primary breast cancers selected for study, the level of receptor activity was related to menopausal status and the tumor content of the specimen. The receptor activity detected varies within a tumor and depends on the tumor content of the biopsy specimen. Predictions based on precise quantitation of receptor concentrations may necessitate replicate tumor sampling and correction for the fraction of nontumor tissue in each sample.

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Accession: 006315209

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PMID: 200253

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