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Reproducibility of micro vascular permeability responses to successive topical applications of brady kinin in the hamster cheek pouch



Reproducibility of micro vascular permeability responses to successive topical applications of brady kinin in the hamster cheek pouch



Microvascular Research 24(3): 354-363



Bradykinin increases leakage of macromolecules at postcapillary venules. Whether the same sites respond to repeated applications of bradykinin is not known. Knowledge of this phenomenon is of importance in ascertaining the mechanisms of vascular wall repair in inflammatory processes. The hamster cheek pouch preparation, suffused with buffer bicarbonate (pH 7.4) was used. Fluorescein isothiocyanate-dextran 150 injected i.v. was used as a tracer of macromolecular microvascular transport. An area of cheek pouch representing 0.8 cm2 was scanned using epi- and transillumination light microscopy before, during and after bradykinin application. Video recordings of individual fields representing 1 mm2 were examined to determine the number of leakage sites present as well as their position in the field. Usually, 20-40 min of suffusion with buffer was sufficient to wash away the effects of any given dose of bradykinin. For fields observed, 85% of the leakage sites induced by the 1st dose were the same sites induced by the 2nd application of the same dose of bradykinin. Whether or not the same site responds to a subsequent application may depend upon the type of microvascular wall repair which ensues. Apparently, relaxation of bradykinin-contracted endothelial cells is a mechanism of microvascular wall repair consistent with those leakage sites which repeatedly respond to bradykinin stimulation; platelet plugging of interendothelial gaps may have occurred in those leakage sites refractory to subsequent applications of bradykinin.

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Accession: 006315212

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PMID: 7154989


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