+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Reproducibility of micro vascular permeability responses to successive topical applications of brady kinin in the hamster cheek pouch

Reproducibility of micro vascular permeability responses to successive topical applications of brady kinin in the hamster cheek pouch

Microvascular Research 24(3): 354-363

Bradykinin increases leakage of macromolecules at postcapillary venules. Whether the same sites respond to repeated applications of bradykinin is not known. Knowledge of this phenomenon is of importance in ascertaining the mechanisms of vascular wall repair in inflammatory processes. The hamster cheek pouch preparation, suffused with buffer bicarbonate (pH 7.4) was used. Fluorescein isothiocyanate-dextran 150 injected i.v. was used as a tracer of macromolecular microvascular transport. An area of cheek pouch representing 0.8 cm2 was scanned using epi- and transillumination light microscopy before, during and after bradykinin application. Video recordings of individual fields representing 1 mm2 were examined to determine the number of leakage sites present as well as their position in the field. Usually, 20-40 min of suffusion with buffer was sufficient to wash away the effects of any given dose of bradykinin. For fields observed, 85% of the leakage sites induced by the 1st dose were the same sites induced by the 2nd application of the same dose of bradykinin. Whether or not the same site responds to a subsequent application may depend upon the type of microvascular wall repair which ensues. Apparently, relaxation of bradykinin-contracted endothelial cells is a mechanism of microvascular wall repair consistent with those leakage sites which repeatedly respond to bradykinin stimulation; platelet plugging of interendothelial gaps may have occurred in those leakage sites refractory to subsequent applications of bradykinin.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 006315212

Download citation: RISBibTeXText

PMID: 7154989

Related references

Responses of pulmonary allo graft and cheek pouch micro vessels to stimulation with histamine and brady kinin and inhibition with a b 2 agonists. Federation Proceedings: Abstract 7168, 1982

Intra vital fluorometric measurements of micro vascular permeability in the hamster cheek pouch. Pfluegers Archiv European Journal of Physiology 389(Suppl.): R13, 1981

Comparison of the action of brady kinin kallidin methionyl lysyl brady kinin and eledoisin on cutaneous vascular permeability. Bulletin of Experimental Biology & Medicine 71(3): 278-280, 1971

The hamster cheek pouch preparation as a model for studies of macro molecular permeability of the micro vasculature. Upsala Journal of Medical Sciences 83(1): 71-79, 1978

Enhancement of the effect of brady kinin on micro vascular permeability by a peptide derived from fibrinogen. Thrombosis & Haemostasis 46(1): 221, 1981

Substance p increase of vascular permeability and presence of nerves of the hamster cheek pouch. Microvascular Research 20(3): 381, 1980

Vascular injury due to topical application of cardiovascular contrast medium in the hamster cheek pouch. Radiology 89(1): 20-23, 1967

Effect of temperature and ph on the responses of micro circulation in hamster cheek pouch 2. Japanese Journal of Pharmacology 33(Suppl.): 298P, 1983

Effect of prostaglandin E1 on leukotriene C4-induced increases in vascular permeability of hamster cheek pouch. Inflammation 16(2): 159-167, 1992

Changes in vascular permeability after local application of roentgen contrast media in the hamster cheek pouch. Acta Radiologica: Diagnosis 11(3): 274-288, 1971

The simultaneous visualization of microspheres and blood flow in the micro vascular bed of the hamster cheek pouch. Microvascular Research 13(2): 203-210, 1977

The effect of calcium on the development of micro vascular leaky sites in the hamster cheek pouch. Federation Proceedings: Abstract 7174, 1982

Application of homologous oxLDL to the hamster cheek pouch microcirculation increased vascular permeability instantly. FASEB Journal 13(5 Part 2): A859, 1999

The effects of catalase, indomethacin and FPL 55712 on vascular permeability in the hamster cheek pouch following scald injury. Prostaglandins Leukotrienes and Medicine 14(3): 297-304, 1984

Vascular permeability and interstitial diffusion of macromolecules in the hamster cheek pouch effects of vasoactive drugs. Federation Proceedings 46(4): 1530, 1987